Region and cell-type resolved quantitative proteomic map of the human heart

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Sophia Doll, Martina Dreßen, Philipp E Geyer, Daniel N Itzhak, Christian Braun, Stefanie A Doppler, Florian Meier, Marcus-Andre Deutsch, Harald Lahm, Rüdiger Lange, Markus Krane, Matthias Mann

The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.

Original languageEnglish
Article number1469
JournalNature Communications
Volume8
Issue number1
Number of pages13
ISSN2041-1723
DOIs
Publication statusPublished - 13 Nov 2017

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  • Journal Article

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