Region and cell-type resolved quantitative proteomic map of the human heart

Research output: Contribution to journalJournal articleResearchpeer-review

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Region and cell-type resolved quantitative proteomic map of the human heart. / Doll, Sophia; Dreßen, Martina; Geyer, Philipp E; Itzhak, Daniel N; Braun, Christian; Doppler, Stefanie A; Meier, Florian; Deutsch, Marcus-Andre; Lahm, Harald; Lange, Rüdiger; Krane, Markus; Mann, Matthias.

In: Nature Communications, Vol. 8, No. 1, 1469, 13.11.2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Doll, S, Dreßen, M, Geyer, PE, Itzhak, DN, Braun, C, Doppler, SA, Meier, F, Deutsch, M-A, Lahm, H, Lange, R, Krane, M & Mann, M 2017, 'Region and cell-type resolved quantitative proteomic map of the human heart', Nature Communications, vol. 8, no. 1, 1469. https://doi.org/10.1038/s41467-017-01747-2

APA

Doll, S., Dreßen, M., Geyer, P. E., Itzhak, D. N., Braun, C., Doppler, S. A., ... Mann, M. (2017). Region and cell-type resolved quantitative proteomic map of the human heart. Nature Communications, 8(1), [1469]. https://doi.org/10.1038/s41467-017-01747-2

Vancouver

Doll S, Dreßen M, Geyer PE, Itzhak DN, Braun C, Doppler SA et al. Region and cell-type resolved quantitative proteomic map of the human heart. Nature Communications. 2017 Nov 13;8(1). 1469. https://doi.org/10.1038/s41467-017-01747-2

Author

Doll, Sophia ; Dreßen, Martina ; Geyer, Philipp E ; Itzhak, Daniel N ; Braun, Christian ; Doppler, Stefanie A ; Meier, Florian ; Deutsch, Marcus-Andre ; Lahm, Harald ; Lange, Rüdiger ; Krane, Markus ; Mann, Matthias. / Region and cell-type resolved quantitative proteomic map of the human heart. In: Nature Communications. 2017 ; Vol. 8, No. 1.

Bibtex

@article{24da4f2458a34d8e95a315c55a937683,
title = "Region and cell-type resolved quantitative proteomic map of the human heart",
abstract = "The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.",
keywords = "Journal Article",
author = "Sophia Doll and Martina Dre{\ss}en and Geyer, {Philipp E} and Itzhak, {Daniel N} and Christian Braun and Doppler, {Stefanie A} and Florian Meier and Marcus-Andre Deutsch and Harald Lahm and R{\"u}diger Lange and Markus Krane and Matthias Mann",
year = "2017",
month = "11",
day = "13",
doi = "10.1038/s41467-017-01747-2",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Region and cell-type resolved quantitative proteomic map of the human heart

AU - Doll, Sophia

AU - Dreßen, Martina

AU - Geyer, Philipp E

AU - Itzhak, Daniel N

AU - Braun, Christian

AU - Doppler, Stefanie A

AU - Meier, Florian

AU - Deutsch, Marcus-Andre

AU - Lahm, Harald

AU - Lange, Rüdiger

AU - Krane, Markus

AU - Mann, Matthias

PY - 2017/11/13

Y1 - 2017/11/13

N2 - The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.

AB - The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.

KW - Journal Article

U2 - 10.1038/s41467-017-01747-2

DO - 10.1038/s41467-017-01747-2

M3 - Journal article

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1469

ER -

ID: 186194379