Recovery of gut microbiota of healthy adults following antibiotic exposure

Research output: Contribution to journalJournal articleResearchpeer-review

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Recovery of gut microbiota of healthy adults following antibiotic exposure. / Palleja, Albert; Mikkelsen, Kristian H; Forslund, Sofia K; Kashani, Alireza; Allin, Kristine H; Nielsen, Trine; Hansen, Tue H; Liang, Suisha; Feng, Qiang; Zhang, Chenchen; Pyl, Paul Theodor; Coelho, Luis Pedro; Yang, Huanming; Wang, Jian; Typas, Athanasios; Nielsen, Morten F.; Nielsen, Henrik Bjorn; Bork, Peer; Wang, Jun; Vilsbøll, Tina; Hansen, Torben; Knop, Filip K; Arumugam, Manimozhiyan; Pedersen, Oluf.

In: Nature Microbiology, Vol. 3, No. 11, 11.2018, p. 1255-1265.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Palleja, A, Mikkelsen, KH, Forslund, SK, Kashani, A, Allin, KH, Nielsen, T, Hansen, TH, Liang, S, Feng, Q, Zhang, C, Pyl, PT, Coelho, LP, Yang, H, Wang, J, Typas, A, Nielsen, MF, Nielsen, HB, Bork, P, Wang, J, Vilsbøll, T, Hansen, T, Knop, FK, Arumugam, M & Pedersen, O 2018, 'Recovery of gut microbiota of healthy adults following antibiotic exposure', Nature Microbiology, vol. 3, no. 11, pp. 1255-1265. https://doi.org/10.1038/s41564-018-0257-9

APA

Palleja, A., Mikkelsen, K. H., Forslund, S. K., Kashani, A., Allin, K. H., Nielsen, T., ... Pedersen, O. (2018). Recovery of gut microbiota of healthy adults following antibiotic exposure. Nature Microbiology, 3(11), 1255-1265. https://doi.org/10.1038/s41564-018-0257-9

Vancouver

Palleja A, Mikkelsen KH, Forslund SK, Kashani A, Allin KH, Nielsen T et al. Recovery of gut microbiota of healthy adults following antibiotic exposure. Nature Microbiology. 2018 Nov;3(11):1255-1265. https://doi.org/10.1038/s41564-018-0257-9

Author

Palleja, Albert ; Mikkelsen, Kristian H ; Forslund, Sofia K ; Kashani, Alireza ; Allin, Kristine H ; Nielsen, Trine ; Hansen, Tue H ; Liang, Suisha ; Feng, Qiang ; Zhang, Chenchen ; Pyl, Paul Theodor ; Coelho, Luis Pedro ; Yang, Huanming ; Wang, Jian ; Typas, Athanasios ; Nielsen, Morten F. ; Nielsen, Henrik Bjorn ; Bork, Peer ; Wang, Jun ; Vilsbøll, Tina ; Hansen, Torben ; Knop, Filip K ; Arumugam, Manimozhiyan ; Pedersen, Oluf. / Recovery of gut microbiota of healthy adults following antibiotic exposure. In: Nature Microbiology. 2018 ; Vol. 3, No. 11. pp. 1255-1265.

Bibtex

@article{63fb0c6a6faa422bb17a9ad30200dbf6,
title = "Recovery of gut microbiota of healthy adults following antibiotic exposure",
abstract = "To minimize the impact of antibiotics, gut microorganisms harbour and exchange antibiotics resistance genes, collectively called their resistome. Using shotgun sequencing-based metagenomics, we analysed the partial eradication and subsequent regrowth of the gut microbiota in 12 healthy men over a 6-month period following a 4-day intervention with a cocktail of 3 last-resort antibiotics: meropenem, gentamicin and vancomycin. Initial changes included blooms of enterobacteria and other pathobionts, such as Enterococcus faecalis and Fusobacterium nucleatum, and the depletion of Bifidobacterium species and butyrate producers. The gut microbiota of the subjects recovered to near-baseline composition within 1.5 months, although 9 common species, which were present in all subjects before the treatment, remained undetectable in most of the subjects after 180 days. Species that harbour β-lactam resistance genes were positively selected for during and after the intervention. Harbouring glycopeptide or aminoglycoside resistance genes increased the odds of de novo colonization, however, the former also decreased the odds of survival. Compositional changes under antibiotic intervention in vivo matched results from in vitro susceptibility tests. Despite a mild yet long-lasting imprint following antibiotics exposure, the gut microbiota of healthy young adults are resilient to a short-term broad-spectrum antibiotics intervention and their antibiotics resistance gene carriage modulates their recovery processes.",
author = "Albert Palleja and Mikkelsen, {Kristian H} and Forslund, {Sofia K} and Alireza Kashani and Allin, {Kristine H} and Trine Nielsen and Hansen, {Tue H} and Suisha Liang and Qiang Feng and Chenchen Zhang and Pyl, {Paul Theodor} and Coelho, {Luis Pedro} and Huanming Yang and Jian Wang and Athanasios Typas and Nielsen, {Morten F.} and Nielsen, {Henrik Bjorn} and Peer Bork and Jun Wang and Tina Vilsb{\o}ll and Torben Hansen and Knop, {Filip K} and Manimozhiyan Arumugam and Oluf Pedersen",
year = "2018",
month = "11",
doi = "10.1038/s41564-018-0257-9",
language = "English",
volume = "3",
pages = "1255--1265",
journal = "Nature Microbiology",
issn = "2058-5276",
publisher = "nature publishing group",
number = "11",

}

RIS

TY - JOUR

T1 - Recovery of gut microbiota of healthy adults following antibiotic exposure

AU - Palleja, Albert

AU - Mikkelsen, Kristian H

AU - Forslund, Sofia K

AU - Kashani, Alireza

AU - Allin, Kristine H

AU - Nielsen, Trine

AU - Hansen, Tue H

AU - Liang, Suisha

AU - Feng, Qiang

AU - Zhang, Chenchen

AU - Pyl, Paul Theodor

AU - Coelho, Luis Pedro

AU - Yang, Huanming

AU - Wang, Jian

AU - Typas, Athanasios

AU - Nielsen, Morten F.

AU - Nielsen, Henrik Bjorn

AU - Bork, Peer

AU - Wang, Jun

AU - Vilsbøll, Tina

AU - Hansen, Torben

AU - Knop, Filip K

AU - Arumugam, Manimozhiyan

AU - Pedersen, Oluf

PY - 2018/11

Y1 - 2018/11

N2 - To minimize the impact of antibiotics, gut microorganisms harbour and exchange antibiotics resistance genes, collectively called their resistome. Using shotgun sequencing-based metagenomics, we analysed the partial eradication and subsequent regrowth of the gut microbiota in 12 healthy men over a 6-month period following a 4-day intervention with a cocktail of 3 last-resort antibiotics: meropenem, gentamicin and vancomycin. Initial changes included blooms of enterobacteria and other pathobionts, such as Enterococcus faecalis and Fusobacterium nucleatum, and the depletion of Bifidobacterium species and butyrate producers. The gut microbiota of the subjects recovered to near-baseline composition within 1.5 months, although 9 common species, which were present in all subjects before the treatment, remained undetectable in most of the subjects after 180 days. Species that harbour β-lactam resistance genes were positively selected for during and after the intervention. Harbouring glycopeptide or aminoglycoside resistance genes increased the odds of de novo colonization, however, the former also decreased the odds of survival. Compositional changes under antibiotic intervention in vivo matched results from in vitro susceptibility tests. Despite a mild yet long-lasting imprint following antibiotics exposure, the gut microbiota of healthy young adults are resilient to a short-term broad-spectrum antibiotics intervention and their antibiotics resistance gene carriage modulates their recovery processes.

AB - To minimize the impact of antibiotics, gut microorganisms harbour and exchange antibiotics resistance genes, collectively called their resistome. Using shotgun sequencing-based metagenomics, we analysed the partial eradication and subsequent regrowth of the gut microbiota in 12 healthy men over a 6-month period following a 4-day intervention with a cocktail of 3 last-resort antibiotics: meropenem, gentamicin and vancomycin. Initial changes included blooms of enterobacteria and other pathobionts, such as Enterococcus faecalis and Fusobacterium nucleatum, and the depletion of Bifidobacterium species and butyrate producers. The gut microbiota of the subjects recovered to near-baseline composition within 1.5 months, although 9 common species, which were present in all subjects before the treatment, remained undetectable in most of the subjects after 180 days. Species that harbour β-lactam resistance genes were positively selected for during and after the intervention. Harbouring glycopeptide or aminoglycoside resistance genes increased the odds of de novo colonization, however, the former also decreased the odds of survival. Compositional changes under antibiotic intervention in vivo matched results from in vitro susceptibility tests. Despite a mild yet long-lasting imprint following antibiotics exposure, the gut microbiota of healthy young adults are resilient to a short-term broad-spectrum antibiotics intervention and their antibiotics resistance gene carriage modulates their recovery processes.

U2 - 10.1038/s41564-018-0257-9

DO - 10.1038/s41564-018-0257-9

M3 - Journal article

VL - 3

SP - 1255

EP - 1265

JO - Nature Microbiology

JF - Nature Microbiology

SN - 2058-5276

IS - 11

ER -

ID: 209358689