Quantifying Diet-Induced Metabolic Changes of the Human Gut Microbiome

Research output: Contribution to journalJournal articleResearchpeer-review

Saeed Shoaie, Pouyan Ghaffari, Petia Kovatcheva-Datchary, Adil Mardinoglu, Partho Sen, Estelle Pujos-Guillot, Tomas de Wouters, Catherine Juste, Salwa Rizkalla, Julien Chilloux, Lesley Hoyles, Jeremy K Nicholson, Joel Dore, Marc E Dumas, Karine Clement, Gert Fredrik Bäckhed, Jens Nielsen, MICRO-Obes Consortium

The human gut microbiome is known to be associated with various human disorders, but a major challenge is to go beyond association studies and elucidate causalities. Mathematical modeling of the human gut microbiome at a genome scale is a useful tool to decipher microbe-microbe, diet-microbe and microbe-host interactions. Here, we describe the CASINO (Community And Systems-level INteractive Optimization) toolbox, a comprehensive computational platform for analysis of microbial communities through metabolic modeling. We first validated the toolbox by simulating and testing the performance of single bacteria and whole communities in vitro. Focusing on metabolic interactions between the diet, gut microbiota, and host metabolism, we demonstrated the predictive power of the toolbox in a diet-intervention study of 45 obese and overweight individuals and validated our predictions by fecal and blood metabolomics data. Thus, modeling could quantitatively describe altered fecal and serum amino acid levels in response to diet intervention.

Original languageEnglish
JournalCell Metabolism
Volume22
Issue number2
Pages (from-to)320-31
Number of pages12
ISSN1550-4131
DOIs
Publication statusPublished - 4 Aug 2015

ID: 150770656