Pulmonary exposure to particles from diesel exhaust, urban dust or single-walled carbon nanotubes and oxidatively damaged DNA and vascular function in apoE(-/-)mice

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Lise K Vesterdal, Kim Jantzen, Majid Sheykhzade, Martin Roursgaard, Janne K Folkmann, Steffen Loft, Peter Møller

Abstract This study compared the oxidative stress level and vasomotor dysfunction after exposure to urban dust, diesel exhaust particles (DEP) or single-walled carbon nanotubes (SWCNT). DEP and SWCNT increased the production of reactive oxygen species (ROS) in cultured endothelial cells and acellullarly, whereas the exposure to urban dust did not generate ROS. ApoE(-/-) mice, which were exposed twice to 0.5 mg/kg of the particles by intratracheal instillation, had unaltered acetylcholine-elicited vasorelaxation in aorta segments. There was unaltered pulmonary expression level of Vcam-1, Icam-1, Hmox-1 and Ogg1. The levels of oxidatively damaged DNA were unchanged in lung tissue. The exposure to SWCNT significantly increased the expression of Ccl-2 in the lung tissue of the mice. The exposure to DEP and SWCNT was associated with elevated ROS production in cultured cells, whereas intratracheal instillation of the same particles had no effect on biomarkers of pulmonary oxidative stress and dilatory dysfunction in the aorta.
Original languageEnglish
JournalNanotoxicology
Volume8
Issue number1
Pages (from-to)61-71
ISSN1743-5390
DOIs
Publication statusPublished - 14 Nov 2012

Bibliographical note

Editor-in-Chief: Prof. Håkan Wallin
2011 Impact Factor: 5.758
5-Year Impact Factor: 6.781
Eight issues are published per year.
ISSN: 1743-5390 (print), 1743-5404 (electronic)

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