Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends. / Hemmingsen, Jette Gjerke; Møller, Peter; Nøjgaard, Jakob Klenø; Roursgaard, Martin; Loft, Steffen.

In: Environmental Science & Technology (Washington), Vol. 45, No. 19, 01.10.2011, p. 8545-51.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hemmingsen, JG, Møller, P, Nøjgaard, JK, Roursgaard, M & Loft, S 2011, 'Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends', Environmental Science & Technology (Washington), vol. 45, no. 19, pp. 8545-51. https://doi.org/10.1021/es200956p

APA

Hemmingsen, J. G., Møller, P., Nøjgaard, J. K., Roursgaard, M., & Loft, S. (2011). Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends. Environmental Science & Technology (Washington), 45(19), 8545-51. https://doi.org/10.1021/es200956p

Vancouver

Hemmingsen JG, Møller P, Nøjgaard JK, Roursgaard M, Loft S. Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends. Environmental Science & Technology (Washington). 2011 Oct 1;45(19):8545-51. https://doi.org/10.1021/es200956p

Author

Hemmingsen, Jette Gjerke ; Møller, Peter ; Nøjgaard, Jakob Klenø ; Roursgaard, Martin ; Loft, Steffen. / Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends. In: Environmental Science & Technology (Washington). 2011 ; Vol. 45, No. 19. pp. 8545-51.

Bibtex

@article{e22bcbda5b934bd2bf35a4d1ef914133,
title = "Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends",
abstract = "Our aim was to compare hazards of particles from combustion of biodiesel blends and conventional diesel (D(100)) in old and improved engines. We determined DNA damage in A549 cells, mRNA levels of CCL2 and IL8 in THP-1 cells, and expression of ICAM-1 and VCAM-1 in human umbilical cord endothelial cells (HUVECs). Viability and production of reactive oxygen species (ROS) were investigated in all cell types. We collected particles from combustion of D(100) and 20{\%} (w/w) blends of animal fat or rapeseed oil methyl esters in light-duty vehicle engines complying with Euro2 or Euro4 standards. Particles emitted from the Euro4 engine were smaller in size and more potent than particles emitted from the Euro2 engine with respect to ROS production and DNA damage, but similarly potent concerning cytokine mRNA expression. Particles emitted from combustion of biodiesel blends were larger in size, and less or equally potent than particles emitted from combustion of D(100) concerning ROS production, DNA damage and mRNA of CCL2 and IL8. ICAM-1 and VCAM-1 expression in HUVECs was only increased by D(100) particles from the Euro4 engine. This suggests that particle emissions from biodiesel in equal mass concentration are less toxic than conventional diesel.",
author = "Hemmingsen, {Jette Gjerke} and Peter M{\o}ller and N{\o}jgaard, {Jakob Klen{\o}} and Martin Roursgaard and Steffen Loft",
year = "2011",
month = "10",
day = "1",
doi = "10.1021/es200956p",
language = "English",
volume = "45",
pages = "8545--51",
journal = "Environmental Science & Technology (Washington)",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "19",

}

RIS

TY - JOUR

T1 - Oxidative stress, genotoxicity, and vascular cell adhesion molecule expression in cells exposed to particulate matter from combustion of conventional diesel and methyl ester biodiesel blends

AU - Hemmingsen, Jette Gjerke

AU - Møller, Peter

AU - Nøjgaard, Jakob Klenø

AU - Roursgaard, Martin

AU - Loft, Steffen

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Our aim was to compare hazards of particles from combustion of biodiesel blends and conventional diesel (D(100)) in old and improved engines. We determined DNA damage in A549 cells, mRNA levels of CCL2 and IL8 in THP-1 cells, and expression of ICAM-1 and VCAM-1 in human umbilical cord endothelial cells (HUVECs). Viability and production of reactive oxygen species (ROS) were investigated in all cell types. We collected particles from combustion of D(100) and 20% (w/w) blends of animal fat or rapeseed oil methyl esters in light-duty vehicle engines complying with Euro2 or Euro4 standards. Particles emitted from the Euro4 engine were smaller in size and more potent than particles emitted from the Euro2 engine with respect to ROS production and DNA damage, but similarly potent concerning cytokine mRNA expression. Particles emitted from combustion of biodiesel blends were larger in size, and less or equally potent than particles emitted from combustion of D(100) concerning ROS production, DNA damage and mRNA of CCL2 and IL8. ICAM-1 and VCAM-1 expression in HUVECs was only increased by D(100) particles from the Euro4 engine. This suggests that particle emissions from biodiesel in equal mass concentration are less toxic than conventional diesel.

AB - Our aim was to compare hazards of particles from combustion of biodiesel blends and conventional diesel (D(100)) in old and improved engines. We determined DNA damage in A549 cells, mRNA levels of CCL2 and IL8 in THP-1 cells, and expression of ICAM-1 and VCAM-1 in human umbilical cord endothelial cells (HUVECs). Viability and production of reactive oxygen species (ROS) were investigated in all cell types. We collected particles from combustion of D(100) and 20% (w/w) blends of animal fat or rapeseed oil methyl esters in light-duty vehicle engines complying with Euro2 or Euro4 standards. Particles emitted from the Euro4 engine were smaller in size and more potent than particles emitted from the Euro2 engine with respect to ROS production and DNA damage, but similarly potent concerning cytokine mRNA expression. Particles emitted from combustion of biodiesel blends were larger in size, and less or equally potent than particles emitted from combustion of D(100) concerning ROS production, DNA damage and mRNA of CCL2 and IL8. ICAM-1 and VCAM-1 expression in HUVECs was only increased by D(100) particles from the Euro4 engine. This suggests that particle emissions from biodiesel in equal mass concentration are less toxic than conventional diesel.

U2 - 10.1021/es200956p

DO - 10.1021/es200956p

M3 - Journal article

VL - 45

SP - 8545

EP - 8551

JO - Environmental Science & Technology (Washington)

JF - Environmental Science & Technology (Washington)

SN - 0013-936X

IS - 19

ER -

ID: 35372791