Mutations in the Kv1.5 channel gene KCNA5 in cardiac arrest patients.

Research output: Contribution to journalJournal articleResearchpeer-review

Nathalie H Nielsen, Bo G Winkel, Jørgen K Kanters, Nicole Schmitt, Jacob Hofman-Bang, Henrik S Jensen, Bo H Bentzen, Bjarne Sigurd, Lars Allan Larsen, Paal S Andersen, Keld Kjeldsen, Morten Grunnet, Michael Christiansen, Søren-Peter Olesen, Stig Haunsø

Mutations in one of the ion channels shaping the cardiac action potential can lead to action potential prolongation. However, only in a minority of cardiac arrest cases mutations in the known arrhythmia-related genes can be identified. In two patients with arrhythmia and cardiac arrest, we identified the point mutations P91L and E33V in the KCNA5 gene encoding the Kv1.5 potassium channel that has not previously been associated with arrhythmia. We functionally characterized the mutations in HEK293 cells. The mutated channels behaved similarly to the wild-type with respect to biophysical characteristics and drug sensitivity. Both patients also carried a D85N polymorphism in KCNE1, which was neither found to influence the Kv1.5 nor the Kv7.1 channel activity. We conclude that although the two N-terminal Kv1.5 mutations did not show any apparent electrophysiological phenotype, it is possible that they may influence other cellular mechanisms responsible for proper electrical behaviour of native cardiomyocytes.
Udgivelsesdato: 2007-Mar-16
Original languageEnglish
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number3
Pages (from-to)776-82
Number of pages6
ISSN0006-291X
DOIs
Publication statusPublished - 2007

Bibliographical note

Keywords: Cells, Cultured; Child; Cloning, Molecular; Electrophysiology; Female; Heart Arrest; Humans; Kv1.5 Potassium Channel; Male; Middle Aged; Mutation; Myocytes, Cardiac; Phenotype; Potassium Channels, Voltage-Gated; Time Factors

ID: 2982889