Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas

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Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas. / Rasmussen, T N; Bersani, M; Schmidt, P; Thim, L; Kofod, Hans; Jørgensen, P N; Poulsen, S S; Holst, J J.

In: Pancreas, Vol. 16, No. 2, 03.1998, p. 195-204.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rasmussen, TN, Bersani, M, Schmidt, P, Thim, L, Kofod, H, Jørgensen, PN, Poulsen, SS & Holst, JJ 1998, 'Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas', Pancreas, vol. 16, no. 2, pp. 195-204.

APA

Rasmussen, T. N., Bersani, M., Schmidt, P., Thim, L., Kofod, H., Jørgensen, P. N., ... Holst, J. J. (1998). Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas. Pancreas, 16(2), 195-204.

Vancouver

Rasmussen TN, Bersani M, Schmidt P, Thim L, Kofod H, Jørgensen PN et al. Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas. Pancreas. 1998 Mar;16(2):195-204.

Author

Rasmussen, T N ; Bersani, M ; Schmidt, P ; Thim, L ; Kofod, Hans ; Jørgensen, P N ; Poulsen, S S ; Holst, J J. / Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas. In: Pancreas. 1998 ; Vol. 16, No. 2. pp. 195-204.

Bibtex

@article{c0d1921074c911dbbee902004c4f4f50,
title = "Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas",
abstract = "The aim of this study was to investigate the possible role of porcine calcitonin gene-related peptide (CGRP) in the regulation of the endocrine porcine pancreas. Initially, we isolated and purified CGRP from extracts of porcine adrenal glands and pancreases. A single molecular form of the peptide was found in the two tissues. The adrenal peptide was sequenced and found to differ from human alpha-CGRP at six positions and from human beta-CGRP at three positions. By immunohistochemistry, CGRP was found in nerve fibers in the pancreatic ganglia. A synthetic replica of the porcine peptide was infused at different dose levels (10(-10), 10(-9), and 10(-8) M) into isolated perfused porcine pancreata. With 5 mmol/L glucose in the perfusate. CGRP at 10(-10) and 10(-9) M increased insulin and glucagon secretion, whereas significant decreases were observed with 10(-8) M. Somatostatin secretion was increased significantly by 10(-8) M CGRP. In immunoneutralization studies (n = 6) using a high-affinity somatostatin antibody, the inhibitory effect of CGRP at 10(-8) M was reversed to a significant stimulation of insulin and glucagon secretion. Insulin secretion in response to square-wave increases in glucose concentration to 11 mM was inhibited dose dependently by CGRP; at 10(-8) M the insulin output decreased by 72+/-9{\%} (n = 6). The present results indicate that CGRP may be involved in the regulation of insulin and glucagon secretion from the porcine pancreas.",
keywords = "Adrenal Glands, Amino Acid Sequence, Animals, Antibodies, Calcitonin Gene-Related Peptide, Glucagon, Humans, Immunohistochemistry, Insulin, Islets of Langerhans, Molecular Sequence Data, Nerve Fibers, Pancreas, Sequence Homology, Somatostatin, Swine",
author = "Rasmussen, {T N} and M Bersani and P Schmidt and L Thim and Hans Kofod and J{\o}rgensen, {P N} and Poulsen, {S S} and Holst, {J J}",
year = "1998",
month = "3",
language = "English",
volume = "16",
pages = "195--204",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams & Wilkins",
number = "2",

}

RIS

TY - JOUR

T1 - Isolation and molecular characterization of porcine calcitonin gene-related peptide (CGRP) and its endocrine effects in the porcine pancreas

AU - Rasmussen, T N

AU - Bersani, M

AU - Schmidt, P

AU - Thim, L

AU - Kofod, Hans

AU - Jørgensen, P N

AU - Poulsen, S S

AU - Holst, J J

PY - 1998/3

Y1 - 1998/3

N2 - The aim of this study was to investigate the possible role of porcine calcitonin gene-related peptide (CGRP) in the regulation of the endocrine porcine pancreas. Initially, we isolated and purified CGRP from extracts of porcine adrenal glands and pancreases. A single molecular form of the peptide was found in the two tissues. The adrenal peptide was sequenced and found to differ from human alpha-CGRP at six positions and from human beta-CGRP at three positions. By immunohistochemistry, CGRP was found in nerve fibers in the pancreatic ganglia. A synthetic replica of the porcine peptide was infused at different dose levels (10(-10), 10(-9), and 10(-8) M) into isolated perfused porcine pancreata. With 5 mmol/L glucose in the perfusate. CGRP at 10(-10) and 10(-9) M increased insulin and glucagon secretion, whereas significant decreases were observed with 10(-8) M. Somatostatin secretion was increased significantly by 10(-8) M CGRP. In immunoneutralization studies (n = 6) using a high-affinity somatostatin antibody, the inhibitory effect of CGRP at 10(-8) M was reversed to a significant stimulation of insulin and glucagon secretion. Insulin secretion in response to square-wave increases in glucose concentration to 11 mM was inhibited dose dependently by CGRP; at 10(-8) M the insulin output decreased by 72+/-9% (n = 6). The present results indicate that CGRP may be involved in the regulation of insulin and glucagon secretion from the porcine pancreas.

AB - The aim of this study was to investigate the possible role of porcine calcitonin gene-related peptide (CGRP) in the regulation of the endocrine porcine pancreas. Initially, we isolated and purified CGRP from extracts of porcine adrenal glands and pancreases. A single molecular form of the peptide was found in the two tissues. The adrenal peptide was sequenced and found to differ from human alpha-CGRP at six positions and from human beta-CGRP at three positions. By immunohistochemistry, CGRP was found in nerve fibers in the pancreatic ganglia. A synthetic replica of the porcine peptide was infused at different dose levels (10(-10), 10(-9), and 10(-8) M) into isolated perfused porcine pancreata. With 5 mmol/L glucose in the perfusate. CGRP at 10(-10) and 10(-9) M increased insulin and glucagon secretion, whereas significant decreases were observed with 10(-8) M. Somatostatin secretion was increased significantly by 10(-8) M CGRP. In immunoneutralization studies (n = 6) using a high-affinity somatostatin antibody, the inhibitory effect of CGRP at 10(-8) M was reversed to a significant stimulation of insulin and glucagon secretion. Insulin secretion in response to square-wave increases in glucose concentration to 11 mM was inhibited dose dependently by CGRP; at 10(-8) M the insulin output decreased by 72+/-9% (n = 6). The present results indicate that CGRP may be involved in the regulation of insulin and glucagon secretion from the porcine pancreas.

KW - Adrenal Glands

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies

KW - Calcitonin Gene-Related Peptide

KW - Glucagon

KW - Humans

KW - Immunohistochemistry

KW - Insulin

KW - Islets of Langerhans

KW - Molecular Sequence Data

KW - Nerve Fibers

KW - Pancreas

KW - Sequence Homology

KW - Somatostatin

KW - Swine

M3 - Journal article

VL - 16

SP - 195

EP - 204

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 2

ER -

ID: 204979