Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)

Research output: Contribution to journalJournal articleResearchpeer-review

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Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber). / Jørgensen, Kristine B.; Krogh-Jensen, Karen; Pickering, Darryl S; Kanui, Titus; Abelson, Klas.

In: Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology, Vol. 202, No. 1, 01.01.2016, p. 7-15.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jørgensen, KB, Krogh-Jensen, K, Pickering, DS, Kanui, T & Abelson, K 2016, 'Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)' Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology, vol. 202, no. 1, pp. 7-15. https://doi.org/10.1007/s00359-015-1048-x

APA

Jørgensen, K. B., Krogh-Jensen, K., Pickering, D. S., Kanui, T., & Abelson, K. (2016). Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber). Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology, 202(1), 7-15. https://doi.org/10.1007/s00359-015-1048-x

Vancouver

Jørgensen KB, Krogh-Jensen K, Pickering DS, Kanui T, Abelson K. Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber). Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology. 2016 Jan 1;202(1):7-15. https://doi.org/10.1007/s00359-015-1048-x

Author

Jørgensen, Kristine B. ; Krogh-Jensen, Karen ; Pickering, Darryl S ; Kanui, Titus ; Abelson, Klas. / Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber). In: Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology. 2016 ; Vol. 202, No. 1. pp. 7-15.

Bibtex

@article{52b6a22521874a24802cdafe98f3eed0,
title = "Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)",
abstract = "The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M1 and M4. The protein sequences for the subtypes m 1–5 of the naked mole-rat were compared to that of the house mouse (Mus musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies with [3H]-N-methylscopolamine. The BLAST test revealed 95 {\%} protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg/kg in the formalin test. Administration of 50 mg/kg VU0152100 resulted in a non-significant tendency towards antinociception. The antinociceptive effects were reversed by the muscarinic acetylcholine receptor antagonist atropine. Binding studies indicated presence of muscarinic acetylcholine receptors with a radioligand affinity comparable to that reported in mice. In conclusion, muscarinic acetylcholine receptor subtypes are present in the naked mole-rat and contribute to antinociception in the naked mole-rat.",
author = "J{\o}rgensen, {Kristine B.} and Karen Krogh-Jensen and Pickering, {Darryl S} and Titus Kanui and Klas Abelson",
year = "2016",
month = "1",
day = "1",
doi = "10.1007/s00359-015-1048-x",
language = "English",
volume = "202",
pages = "7--15",
journal = "Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology",
issn = "0340-7594",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)

AU - Jørgensen, Kristine B.

AU - Krogh-Jensen, Karen

AU - Pickering, Darryl S

AU - Kanui, Titus

AU - Abelson, Klas

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M1 and M4. The protein sequences for the subtypes m 1–5 of the naked mole-rat were compared to that of the house mouse (Mus musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies with [3H]-N-methylscopolamine. The BLAST test revealed 95 % protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg/kg in the formalin test. Administration of 50 mg/kg VU0152100 resulted in a non-significant tendency towards antinociception. The antinociceptive effects were reversed by the muscarinic acetylcholine receptor antagonist atropine. Binding studies indicated presence of muscarinic acetylcholine receptors with a radioligand affinity comparable to that reported in mice. In conclusion, muscarinic acetylcholine receptor subtypes are present in the naked mole-rat and contribute to antinociception in the naked mole-rat.

AB - The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M1 and M4. The protein sequences for the subtypes m 1–5 of the naked mole-rat were compared to that of the house mouse (Mus musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies with [3H]-N-methylscopolamine. The BLAST test revealed 95 % protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg/kg in the formalin test. Administration of 50 mg/kg VU0152100 resulted in a non-significant tendency towards antinociception. The antinociceptive effects were reversed by the muscarinic acetylcholine receptor antagonist atropine. Binding studies indicated presence of muscarinic acetylcholine receptors with a radioligand affinity comparable to that reported in mice. In conclusion, muscarinic acetylcholine receptor subtypes are present in the naked mole-rat and contribute to antinociception in the naked mole-rat.

U2 - 10.1007/s00359-015-1048-x

DO - 10.1007/s00359-015-1048-x

M3 - Journal article

VL - 202

SP - 7

EP - 15

JO - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology

JF - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology

SN - 0340-7594

IS - 1

ER -

ID: 148102729