Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle

Research output: Contribution to journalJournal articleResearchpeer-review

Abdullah A Al-Shammari, Roger W P Kissane, Simon Holbek, Abigail Louise Mackey, Thomas Rostgaard Andersen, Eamonn A Gaffney, Michael Kjær, Stuart Egginton

Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.

Original languageEnglish
JournalJournal of Applied Physiology
Issue number3
Pages (from-to)544-557
Number of pages14
Publication statusPublished - 2019

    Research areas

  • The Faculty of Science - Mathematical modeling, Skeletal muscle, Image analysis, Fiber type, Capillary supply, DTect

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