Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

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Daniel J Fazakerley, Andreas Mæchel Fritzen, Marin E Nelson, Ida H Thorius, Kristen C Cooke, Sean J Humphrey, Gregory J Cooney, David E James

Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
Original languageEnglish
Article numbere3146
JournalBio-protocol
Volume9
Issue number2
Number of pages13
ISSN2331-8325
DOIs
Publication statusPublished - 2019

    Research areas

  • The Faculty of Science - Insulin, Adipose tissue, Muscle, Glucose transport, Glucose uptake, Insulin tolerance test

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