Inhibition by indomethacin and aspirin of 15 hydroxy prostaglandin dehydrogenase in vitro
Research output: Contribution to journal › Journal article › Research › peer-review
15 Hydroxyprostaglandin dehydrogenase from bovine lung was purified 7.4 times to a specific activity of 1.4 mU/mg of protein. The isoelectric point was estimated at 5.4 and the molecular weight by gel filtration at 40,000. K(m) for prostaglandin E and for NAD was found to be 3.4 µM and 1.1 x 10M respectively. The enzyme was inhibited by indomethacin and aspirin. The indomethacin inhibition was found to be non competitive to prostaglandin E having a K(i) = 1.4 x 10M and a K'(i) = 1.6 x 10M.
|Journal||Prostaglandins - Biology|
|Number of pages||11|
|Publication status||Published - 1 Jan 1974|