Increased capillary density in skeletal muscle is not associated with impaired insulin sensitivity induced by bed rest in healthy young men

Research output: Contribution to journalJournal articleResearchpeer-review

David Montero, Laura Oberholzer, Thomas Haider, Andreas Breenfeldt Andersen, Sune Dandanell, Anne-Kristine Meinild-Lundby, Hannah Maconochie, Carsten Lundby

Physical inactivity alters glucose homeostasis in skeletal muscle, potentially developing into overt metabolic disease. The present study sought to investigate the role of skeletal muscle capillarization in glucose tolerance and insulin sensitivity (IS) using a classic human model of physical inactivity. Thirteen healthy males (age=23±2 yr) underwent 4 days of full-time supervised and diet-controlled bed rest. Oral glucose tolerance test (OGTT), indices of insulin sensitivity (IS) (quantitative IS check index (QUICKI), Matsuda index) as well as skeletal muscle biopsies with measurement of fiber type distribution, fiber cross-sectional area (FCSA), capillary-to-fiber ratio (C/F ratio) and capillary density (CD) were assessed prior to and after bed rest. Body weight and composition were unaltered by bed rest. Fasting glucose/insulin ratio (G0/I0 ratio) (-25 %, P=0.016), QUICKI (-7 %, P=0.023) and Matsuda index (-24 %, P=0.003) diminished with bed rest. Skeletal muscle FCSA decreased (-737.4±763.2 µm-2 (-12 %), P=0.005) while C/F ratio was preserved, resulting in augmented CD (+93.9±91.5 capillaries•mm-2 (+37 %), P=0.003) with bed rest. No association was detected between changes in skeletal muscle variables and metabolic outcomes. Independently of bed rest-induced effects, a positive linear relationship was detected between C/F ratio and G0/I0 ratio (β=17.09, P=0.021). In conclusion, impaired glucose homeostasis with bed rest is not prevented nor associated with enhanced skeletal muscle capillarization in healthy individuals.

Original languageEnglish
JournalApplied Physiology, Nutrition and Metabolism
Volume43
Issue number12
Pages (from-to)1334-1340
Number of pages7
ISSN1715-5312
DOIs
Publication statusPublished - 2018

    Research areas

  • The Faculty of Science - Glucose tolerance, Insulin resistance, Skeletal muscle capillarization, Bed rest

ID: 201832351