Human muscular mitochondrial fusion in athletes during exercise
Research output: Contribution to journal › Journal article › Research › peer-review
Jesús R Huertas, Francisco Javier Ruiz-Ojeda, Julio Plaza-Díaz, Nikolai Baastrup Nordsborg, Jesús Martín-Albo, Ascensión Rueda-Robles, Rafael A Casuso
The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from M. triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology.
|Journal||F A S E B Journal|
|Publication status||E-pub ahead of print - 9 Aug 2019|
- The Faculty of Science - Physical activity, Mitochondrial fission, Skeletal muscle, Mitofusin 2