Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain.

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Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain. / Adari, H; Lowy, D R; Willumsen, B M; Der, C J; McCormick, F.

In: Science, Vol. 240, No. 4851, 1988, p. 518-21.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Adari, H, Lowy, DR, Willumsen, BM, Der, CJ & McCormick, F 1988, 'Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain.', Science, vol. 240, no. 4851, pp. 518-21.

APA

Adari, H., Lowy, D. R., Willumsen, B. M., Der, C. J., & McCormick, F. (1988). Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain. Science, 240(4851), 518-21.

Vancouver

Adari H, Lowy DR, Willumsen BM, Der CJ, McCormick F. Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain. Science. 1988;240(4851):518-21.

Author

Adari, H ; Lowy, D R ; Willumsen, B M ; Der, C J ; McCormick, F. / Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain. In: Science. 1988 ; Vol. 240, No. 4851. pp. 518-21.

Bibtex

@article{e0d30b80e3b711dcbee902004c4f4f50,
title = "Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain.",
abstract = "A cytoplasmic protein that greatly enhances the guanosine triphosphatase (GTPase) activity of N-ras protein but does not affect the activity of oncogenic ras mutants has been recently described. This protein (GAP) is shown here to be ubiquitous in higher eukaryotes and to interact with H-ras as well as with N-ras proteins. To identify the region of ras p21 with which GAP interacts, 21 H-ras mutant proteins were purified and tested for their ability to undergo stimulation of GTPase activity by GAP. Mutations in nonessential regions of H-ras p21 as well as mutations in its carboxyl-terminal domain (residues 165-185) and purine binding region (residues 117 and 119) did not decrease the ability of the protein to respond to GAP. In addition, an antibody against the carboxyl-terminal domain did not block GAP activity, supporting the conclusion that GAP does not interact with this region. Transforming mutations at positions 12, 59, and 61 (the phosphoryl binding region) abolished GTPase stimulation by GAP. Point mutations in the putative effector region of ras p21 (amino acids 35, 36, and 38) were also insensitive to GAP. However, a point mutation at position 39, shown previously not to impair effector function, did not alter GAP-p21 interaction. These results indicate that GAP interaction may be essential for ras p21 biological activity and that it may be a ras effector protein.",
author = "H Adari and Lowy, {D R} and Willumsen, {B M} and Der, {C J} and F McCormick",
note = "Keywords: Amino Acid Sequence; Animals; Antibodies, Monoclonal; DNA Mutational Analysis; Enzyme Activation; GTP Phosphohydrolases; GTP-Binding Proteins; GTPase-Activating Proteins; Genes, ras; Immunologic Techniques; Phosphoric Monoester Hydrolases; Proteins; Proto-Oncogene Proteins; Structure-Activity Relationship; ras GTPase-Activating Proteins",
year = "1988",
language = "English",
volume = "240",
pages = "518--21",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4851",

}

RIS

TY - JOUR

T1 - Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain.

AU - Adari, H

AU - Lowy, D R

AU - Willumsen, B M

AU - Der, C J

AU - McCormick, F

N1 - Keywords: Amino Acid Sequence; Animals; Antibodies, Monoclonal; DNA Mutational Analysis; Enzyme Activation; GTP Phosphohydrolases; GTP-Binding Proteins; GTPase-Activating Proteins; Genes, ras; Immunologic Techniques; Phosphoric Monoester Hydrolases; Proteins; Proto-Oncogene Proteins; Structure-Activity Relationship; ras GTPase-Activating Proteins

PY - 1988

Y1 - 1988

N2 - A cytoplasmic protein that greatly enhances the guanosine triphosphatase (GTPase) activity of N-ras protein but does not affect the activity of oncogenic ras mutants has been recently described. This protein (GAP) is shown here to be ubiquitous in higher eukaryotes and to interact with H-ras as well as with N-ras proteins. To identify the region of ras p21 with which GAP interacts, 21 H-ras mutant proteins were purified and tested for their ability to undergo stimulation of GTPase activity by GAP. Mutations in nonessential regions of H-ras p21 as well as mutations in its carboxyl-terminal domain (residues 165-185) and purine binding region (residues 117 and 119) did not decrease the ability of the protein to respond to GAP. In addition, an antibody against the carboxyl-terminal domain did not block GAP activity, supporting the conclusion that GAP does not interact with this region. Transforming mutations at positions 12, 59, and 61 (the phosphoryl binding region) abolished GTPase stimulation by GAP. Point mutations in the putative effector region of ras p21 (amino acids 35, 36, and 38) were also insensitive to GAP. However, a point mutation at position 39, shown previously not to impair effector function, did not alter GAP-p21 interaction. These results indicate that GAP interaction may be essential for ras p21 biological activity and that it may be a ras effector protein.

AB - A cytoplasmic protein that greatly enhances the guanosine triphosphatase (GTPase) activity of N-ras protein but does not affect the activity of oncogenic ras mutants has been recently described. This protein (GAP) is shown here to be ubiquitous in higher eukaryotes and to interact with H-ras as well as with N-ras proteins. To identify the region of ras p21 with which GAP interacts, 21 H-ras mutant proteins were purified and tested for their ability to undergo stimulation of GTPase activity by GAP. Mutations in nonessential regions of H-ras p21 as well as mutations in its carboxyl-terminal domain (residues 165-185) and purine binding region (residues 117 and 119) did not decrease the ability of the protein to respond to GAP. In addition, an antibody against the carboxyl-terminal domain did not block GAP activity, supporting the conclusion that GAP does not interact with this region. Transforming mutations at positions 12, 59, and 61 (the phosphoryl binding region) abolished GTPase stimulation by GAP. Point mutations in the putative effector region of ras p21 (amino acids 35, 36, and 38) were also insensitive to GAP. However, a point mutation at position 39, shown previously not to impair effector function, did not alter GAP-p21 interaction. These results indicate that GAP interaction may be essential for ras p21 biological activity and that it may be a ras effector protein.

M3 - Journal article

VL - 240

SP - 518

EP - 521

JO - Science

JF - Science

SN - 0036-8075

IS - 4851

ER -

ID: 2890894