Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells. / Skytt, D. M.; Toft-Kehler, A. K.; Braendstrup, C. T.; Cejvanovic, S; Gurubaran, I. S.; Bergersen, L. H.; Kolko, M.

In: Journal of Biomedicine and Biotechnology, Vol. 2016, 1087647, 2016.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Skytt, DM, Toft-Kehler, AK, Braendstrup, CT, Cejvanovic, S, Gurubaran, IS, Bergersen, LH & Kolko, M 2016, 'Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells', Journal of Biomedicine and Biotechnology, vol. 2016, 1087647. https://doi.org/10.1155/2016/1087647

APA

Skytt, D. M., Toft-Kehler, A. K., Braendstrup, C. T., Cejvanovic, S., Gurubaran, I. S., Bergersen, L. H., & Kolko, M. (2016). Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells. Journal of Biomedicine and Biotechnology, 2016, [1087647]. https://doi.org/10.1155/2016/1087647

Vancouver

Skytt DM, Toft-Kehler AK, Braendstrup CT, Cejvanovic S, Gurubaran IS, Bergersen LH et al. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells. Journal of Biomedicine and Biotechnology. 2016;2016. 1087647. https://doi.org/10.1155/2016/1087647

Author

Skytt, D. M. ; Toft-Kehler, A. K. ; Braendstrup, C. T. ; Cejvanovic, S ; Gurubaran, I. S. ; Bergersen, L. H. ; Kolko, M. / Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells. In: Journal of Biomedicine and Biotechnology. 2016 ; Vol. 2016.

Bibtex

@article{9eef70c032704bd78ffda30e465825c9,
title = "Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells",
abstract = "Glia-neuron partnership is important for inner retinal homeostasis and any disturbances may result in retinal ganglion cell (RGC) death. M{\"u}ller cells support RGCs with essential functions such as removing excess glutamate and providing energy sources. The aim was to explore the impact of M{\"u}ller cells on RGC survival. To investigate the M{\"u}ller cell/RGC interactions we developed a coculture model, in which primary M{\"u}ller cells were grown in inserts on top of pure primary RGC cultures. The impact of starvation and mitochondrial inhibition on the M{\"u}ller cell ability to protect RGCs was studied. Moreover, the ability of M{\"u}ller cells to remove glutamate from the extracellular space was investigated. RGC survival was evaluated by cell viability assays and glutamate uptake was assessed by kinetic uptake assays. We demonstrated a significantly increased RGC survival in presence of untreated and prestarved M{\"u}ller cells. Additionally, prestarved M{\"u}ller cells significantly increased RGC survival after mitochondrial inhibition. Finally, we revealed a significantly increased ability to take up glutamate in starved M{\"u}ller cells. Overall, our study confirms essential roles of M{\"u}ller cells in RGC survival. We suggest that targeting M{\"u}ller cell function could have potential for future treatment strategies to prevent blinding neurodegenerative retinal diseases.",
author = "Skytt, {D. M.} and Toft-Kehler, {A. K.} and Braendstrup, {C. T.} and S Cejvanovic and Gurubaran, {I. S.} and Bergersen, {L. H.} and M. Kolko",
year = "2016",
doi = "10.1155/2016/1087647",
language = "English",
volume = "2016",
journal = "Journal of Biomedicine and Biotechnology",
issn = "1110-7243",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Muller Cells and Retinal Ganglion Cells

AU - Skytt, D. M.

AU - Toft-Kehler, A. K.

AU - Braendstrup, C. T.

AU - Cejvanovic, S

AU - Gurubaran, I. S.

AU - Bergersen, L. H.

AU - Kolko, M.

PY - 2016

Y1 - 2016

N2 - Glia-neuron partnership is important for inner retinal homeostasis and any disturbances may result in retinal ganglion cell (RGC) death. Müller cells support RGCs with essential functions such as removing excess glutamate and providing energy sources. The aim was to explore the impact of Müller cells on RGC survival. To investigate the Müller cell/RGC interactions we developed a coculture model, in which primary Müller cells were grown in inserts on top of pure primary RGC cultures. The impact of starvation and mitochondrial inhibition on the Müller cell ability to protect RGCs was studied. Moreover, the ability of Müller cells to remove glutamate from the extracellular space was investigated. RGC survival was evaluated by cell viability assays and glutamate uptake was assessed by kinetic uptake assays. We demonstrated a significantly increased RGC survival in presence of untreated and prestarved Müller cells. Additionally, prestarved Müller cells significantly increased RGC survival after mitochondrial inhibition. Finally, we revealed a significantly increased ability to take up glutamate in starved Müller cells. Overall, our study confirms essential roles of Müller cells in RGC survival. We suggest that targeting Müller cell function could have potential for future treatment strategies to prevent blinding neurodegenerative retinal diseases.

AB - Glia-neuron partnership is important for inner retinal homeostasis and any disturbances may result in retinal ganglion cell (RGC) death. Müller cells support RGCs with essential functions such as removing excess glutamate and providing energy sources. The aim was to explore the impact of Müller cells on RGC survival. To investigate the Müller cell/RGC interactions we developed a coculture model, in which primary Müller cells were grown in inserts on top of pure primary RGC cultures. The impact of starvation and mitochondrial inhibition on the Müller cell ability to protect RGCs was studied. Moreover, the ability of Müller cells to remove glutamate from the extracellular space was investigated. RGC survival was evaluated by cell viability assays and glutamate uptake was assessed by kinetic uptake assays. We demonstrated a significantly increased RGC survival in presence of untreated and prestarved Müller cells. Additionally, prestarved Müller cells significantly increased RGC survival after mitochondrial inhibition. Finally, we revealed a significantly increased ability to take up glutamate in starved Müller cells. Overall, our study confirms essential roles of Müller cells in RGC survival. We suggest that targeting Müller cell function could have potential for future treatment strategies to prevent blinding neurodegenerative retinal diseases.

U2 - 10.1155/2016/1087647

DO - 10.1155/2016/1087647

M3 - Journal article

VL - 2016

JO - Journal of Biomedicine and Biotechnology

JF - Journal of Biomedicine and Biotechnology

SN - 1110-7243

M1 - 1087647

ER -

ID: 166663672