Fractional 13C enrichment of isolated carbons using [1-13C]- or [2- 13C]-glucose facilitates the accurate measurement of dynamics at backbone Calpha and side-chain methyl positions in proteins

Research output: Contribution to journalJournal articleResearchpeer-review

Patrik Lundström, Kaare Teilum, Tommy Carstensen, Irina Bezsonova, Silke Wiesner, D Flemming Hansen, Tomasz L Religa, Mikael Akke, Lewis E Kay

A simple labeling approach is presented based on protein expression in [1-(13)C]- or [2-(13)C]-glucose containing media that produces molecules enriched at methyl carbon positions or backbone C(alpha) sites, respectively. All of the methyl groups, with the exception of Thr and Ile(delta1) are produced with isolated (13)C spins (i.e., no (13)C-(13)C one bond couplings), facilitating studies of dynamics through the use of spin-spin relaxation experiments without artifacts introduced by evolution due to large homonuclear scalar couplings. Carbon-alpha sites are labeled without concomitant labeling at C(beta) positions for 17 of the common 20 amino acids and there are no cases for which (13)C(alpha)-(13)CO spin pairs are observed. A large number of probes are thus available for the study of protein dynamics with the results obtained complimenting those from more traditional backbone (15)N studies. The utility of the labeling is established by recording (13)C R (1rho) and CPMG-based experiments on a number of different protein systems.
Original languageEnglish
JournalJournal of Biomolecular NMR
Volume38
Issue number3
Pages (from-to)199-212
Number of pages13
ISSN0925-2738
DOIs
Publication statusPublished - 2007
Externally publishedYes

Bibliographical note

Keywords: Amino Acid Sequence; Amino Acids; Animals; Carbon; Carbon Isotopes; Cattle; Glucose; Humans; Methane; Nuclear Magnetic Resonance, Biomolecular; Proteins

ID: 15288472