Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

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Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare. / Jensen, Tanja L.; Kiersgaard, Maria K.; Mikkelsen, Lars F.; Sørensen, Dorte B.

In: Laboratory Animals, 01.01.2019.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jensen, TL, Kiersgaard, MK, Mikkelsen, LF & Sørensen, DB 2019, 'Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare' Laboratory Animals. https://doi.org/10.1177/0023677218824373

APA

Jensen, T. L., Kiersgaard, M. K., Mikkelsen, L. F., & Sørensen, D. B. (2019). Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare. Laboratory Animals. https://doi.org/10.1177/0023677218824373

Vancouver

Jensen TL, Kiersgaard MK, Mikkelsen LF, Sørensen DB. Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare. Laboratory Animals. 2019 Jan 1. https://doi.org/10.1177/0023677218824373

Author

Jensen, Tanja L. ; Kiersgaard, Maria K. ; Mikkelsen, Lars F. ; Sørensen, Dorte B. / Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare. In: Laboratory Animals. 2019.

Bibtex

@article{a56aaf0a79124d7d8d749a60afbe8ee7,
title = "Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare",
abstract = "Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.",
keywords = "Fasting, mouse, refinement, welfare",
author = "Jensen, {Tanja L.} and Kiersgaard, {Maria K.} and Mikkelsen, {Lars F.} and S{\o}rensen, {Dorte B.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1177/0023677218824373",
language = "English",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications",

}

RIS

TY - JOUR

T1 - Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

AU - Jensen, Tanja L.

AU - Kiersgaard, Maria K.

AU - Mikkelsen, Lars F.

AU - Sørensen, Dorte B.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.

AB - Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.

KW - Fasting

KW - mouse

KW - refinement

KW - welfare

UR - http://www.scopus.com/inward/record.url?scp=85061611693&partnerID=8YFLogxK

U2 - 10.1177/0023677218824373

DO - 10.1177/0023677218824373

M3 - Journal article

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

ER -

ID: 217946377