Evidence for an association between the Leu162Val polymorphism of the PPARalpha gene and decreased fasting serum triglyceride levels in glucose tolerant subjects

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Eva-Maria D Nielsen, Lars Hansen, Søren Morgenthaler Echwald, Thomas Drivsholm, Knut Borch-Johnsen, Claus Thorn Ekstrøm, Torben Hansen, Oluf Pedersen

The aim of the study was to investigate whether genetic variation in the peroxisome proliferator-activated receptor-alpha (PPARalpha) is associated with type 2 diabetes and altered lipid or carbohydrate metabolism in glucose tolerant subjects. Mutation analyses of PPARalpha were performed in 56 type 2 diabetic patients. Six variants were identified: IVS3 + 76T>C, IVS3-19C>T, IVS4 + 35C>T, Leu162Val, Arg178Gly and Ala268Val. In a case-control study comprising 738 type 2 diabetic patients and 524 glucose tolerant subjects, the three exon variants did not show any significant differences in allele frequencies between type 2 diabetic patients and control subjects. The functional Leu162Val polymorphism was further investigated in genotype-phenotype studies involving 340 young, healthy subjects and 502 middle-aged glucose tolerant subjects. The young, healthy subjects who were heterozygous for the Leu162Val variant had, on average, a 20% decrease in fasting serum triglyceride levels (P=0.014). This finding was replicated in middle-aged subjects (P=0.023). The Leu162Val polymorphism was not related to alterations in insulin sensitivity, insulin release or level of glycaemia. In conclusion, the Leu162Val polymorphism of PPARalpha is associated with a decreased level of fasting serum triglyceride in glucose tolerant white subjects.
Original languageEnglish
JournalPharmacogenetics and Genomics
Volume13
Issue number7
Pages (from-to)417-23
Number of pages7
ISSN1744-6872
DOIs
Publication statusPublished - 2003

    Research areas

  • Adult, Aged, Alleles, Blood Glucose, Body Constitution, Case-Control Studies, DNA Mutational Analysis, Denmark, Diabetes Mellitus, Type 2, European Continental Ancestry Group, Fasting, Female, Gene Frequency, Genetic Variation, Glucose Tolerance Test, Heterozygote, Humans, Insulin, Male, Middle Aged, Point Mutation, Polymorphism, Genetic, Receptors, Cytoplasmic and Nuclear, Transcription Factors, Triglycerides, Valine

ID: 38336644