Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

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Heidi Ugelstad Eirheim, Christoffer Bundgaard, Hanne Mørck Nielsen

The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P
Original languageEnglish
JournalToxicology in Vitro
Issue number5
Pages (from-to)649-57
Number of pages9
Publication statusPublished - 2004

    Research areas

  • Adenosine Triphosphate, Cell Line, Tumor, Cell Membrane Permeability, Cell Proliferation, Detergents, Dose-Response Relationship, Drug, Epithelial Cells, Glycocholic Acid, Humans, Inhibitory Concentration 50, Mannitol, Methylphenazonium Methosulfate, Mouth Mucosa, Neutral Red, Proteins, Reproducibility of Results, Tetrazolium Salts, Thiazoles, Toxicity Tests

ID: 43890885