Effects of PYY3-36 and GLP-1 on energy intake, energy expenditure and appetite in overweight men

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Julie Berg Schmidt, Nikolaj Ture Gregersen, Sue D Pedersen, Johanne L Arentoft, Christian Ritz, Thue W. Schwartz, Jens Juul Holst, Arne Astrup, Anders Mikael Sjödin

Aim: To examine the effects of GLP-1 and PYY3-36, separately and in combination, on energy intake, energy expenditure, appetite sensations, glucose and fat metabolism, ghrelin and vital signs in healthy overweight men.
Methods: 25 healthy, male subjects participated in this randomized, double-blinded, placebo-controlled 4-arm crossover study (BMI:29±3 kg/m2, age:33±9 years). On separate days they received a 150 min intravenous infusion of either a) 0.8pmol/kg/min PYY3-36, b) 1.0 pmol/kg/min GLP-1, c) a+b, or d) placebo. Ad libitum energy intake was assessed during the final 30 min. Measurements of appetite sensations, energy expenditure and fat oxidation, vital signs and blood variables were collected throughout the infusion period.
Results: No effect on energy intake was found after monoinfusions of PYY3-36 (-4.2±4.8%, P=0.8) or GLP-1 (-3.0±4.5%, P=0.9). However, the co-infusion reduced energy intake compared to placebo (-30.4±6.5%, P<0.0001) and more than the sum of the monoinfusions (P<0.001), demonstrating a synergistic effect. Co-infusion slightly increased sensation of nausea (P<0.05), but this effect could not explain the effect on energy intake. A decrease in plasma ghrelin was found after all treatments compared to placebo (all P<0.05); however, infusions of GLP-1+PYY3-36 resulted in an additional decrease compared to the monoinfusions (both P<0.01).
Conclusion: Co-infusion of GLP-1 and PYY3-36 exerted a synergistic effect on energy intake. The satiating effect of the meal was enhanced by GLP-1 and PYY3-36 in combination compared to placebo. Co-infusion was accompanied by slightly increased nausea and a decrease in plasma ghrelin, but neither of these
factors could explain the reduction in energy intake.
Original languageEnglish
JournalAmerican Journal of Physiology: Endocrinology and Metabolism
Volume306
Issue number11
Pages (from-to)E1248-E1256
Number of pages9
ISSN0193-1849
DOIs
Publication statusPublished - 2014

ID: 107926442