Disruption of endocytic trafficking in frontotemporal dementia with CHMP2B mutations

Research output: Contribution to journalJournal articleResearchpeer-review

Hazel Urwin, Astrid Authier, Jørgen Erik Nielsen, Daniel Metcalf, Caroline Powell, Kristina Froud, Denise S Malcolm, Ida Holm, Peter Johannsen, Jeremy Brown, Elizabeth M C Fisher, Julie van der Zee, Marc Bruyland, FReJA Consortium, Christine Van Broeckhoven, John Collinge, Sebastian Brandner, Clare Futter, Adrian M Isaacs

Mutations in CHMP2B cause frontotemporal dementia (FTD) in a large Danish pedigree, which is termed FTD linked to chromosome 3 (FTD-3), and also in an unrelated familial FTD patient. CHMP2B is a component of the ESCRT-III complex, which is required for function of the multivesicular body (MVB), an endosomal structure that fuses with the lysosome to degrade endocytosed proteins. We report a novel endosomal pathology in CHMP2B mutation-positive patient brains and also identify and characterize abnormal endosomes in patient fibroblasts. Functional studies demonstrate a specific disruption of endosome-lysosome fusion but not protein sorting by the MVB. We provide evidence for a mechanism for impaired endosome-lysosome fusion whereby mutant CHMP2B constitutively binds to MVBs and prevents recruitment of proteins necessary for fusion to occur, such as Rab7. The fusion of endosomes with lysosomes is required for neuronal function and the data presented therefore suggest a pathogenic mechanism for FTD caused by CHMP2B mutations.
Original languageEnglish
JournalHuman Molecular Genetics
Volume19
Issue number11
Pages (from-to)2228-38
Number of pages10
ISSN0964-6906
DOIs
Publication statusPublished - 2010

ID: 20970311