Dichotomy of the human T cell response to Leishmania antigens. II. Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients

Research output: Contribution to journalJournal articleResearchpeer-review

J A Kurtzhals, A S Hey, A Jardim, M Kemp, K U Schaefer, E O Odera, C B Christensen, J I Githure, R W Olafson, T G Theander

The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) production in PBMC from cured patients, while cells from non-exposed donors gave weak responses. A similar pattern was induced by lipophosphoglycan-associated protein (LPGAP). By contrast, the major surface protease of Leishmania, gp63, induced only a weak proliferative response without IFN-gamma production in five of 17 samples from cured patients. Four of the five responding cultures produced IL-4, i.e. the response to this antigen was of the Th2 type. Furthermore, sera from acutely ill visceral leishmaniasis patients contained high levels of IgG antibodies to gp63. The Th2-like response to gp63 in patients cured of visceral leishmaniasis differs from the Th1-like response to the same antigen observed in patients cured of cutaneous leishmaniasis.
Original languageEnglish
JournalClinical and Experimental Immunology
Volume96
Issue number3
Pages (from-to)416-21
Number of pages5
ISSN0009-9104
Publication statusPublished - 1994

Bibliographical note

Keywords: Animals; Antibodies, Protozoan; Antigens, Protozoan; Glycosphingolipids; Humans; Interferon Type II; Interleukin-4; Leishmania donovani; Leishmaniasis, Visceral; Lymphocyte Activation; Metalloendopeptidases; Protozoan Proteins; T-Lymphocyte Subsets; T-Lymphocytes; T-Lymphocytes, Helper-Inducer

ID: 6766599