Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus

Research output: Contribution to journalJournal articleResearchpeer-review

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Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus. / Adhikary, Sadhana; Bisgaard, Magne; Boot, Ron; Benga, Laurentiu; Nicklas, Werner; Christensen, Henrik.

In: Veterinary Microbiology, Vol. 231, 2019, p. 11-17.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Adhikary, S, Bisgaard, M, Boot, R, Benga, L, Nicklas, W & Christensen, H 2019, 'Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus', Veterinary Microbiology, vol. 231, pp. 11-17. https://doi.org/10.1016/j.vetmic.2019.02.025

APA

Adhikary, S., Bisgaard, M., Boot, R., Benga, L., Nicklas, W., & Christensen, H. (2019). Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus. Veterinary Microbiology, 231, 11-17. https://doi.org/10.1016/j.vetmic.2019.02.025

Vancouver

Adhikary S, Bisgaard M, Boot R, Benga L, Nicklas W, Christensen H. Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus. Veterinary Microbiology. 2019;231:11-17. https://doi.org/10.1016/j.vetmic.2019.02.025

Author

Adhikary, Sadhana ; Bisgaard, Magne ; Boot, Ron ; Benga, Laurentiu ; Nicklas, Werner ; Christensen, Henrik. / Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus. In: Veterinary Microbiology. 2019 ; Vol. 231. pp. 11-17.

Bibtex

@article{495d639336034f18a590ab1042009fb7,
title = "Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus",
abstract = "The aim of the investigation was to develop a definitive typing system for Rodentibacter pneumotropicus. A total of 79 strains including the type strain of R. pneumotropicus, all associated with rodents were used to develop a multi-locus sequence typing scheme (MLST). Primers were designed for conserved regions of seven house-keeping genes (atpG, frdB, gdh, pgi, pmi, recA, zwf) and internal fragments of 399–839 bp were sequenced for all strains. The genes were also extracted in full length from whole genomic sequences of 14 strains of which 10 were sequenced in the current study. The number of alleles at the different loci ranged from 5 to 7 and a total of 20 allelic profiles or sequence types were recognized amongst the 79 strains. Analysis of the MLST data showed that some STs have been stable over many years probably circulating in the same colonies and probably transferred between colonies. We assume that this MLST scheme may provide a high level of resolution and might be an excellent tool for studying the population structure and epidemiology of R. pneumotropicus. Further development of the scheme is expected by including more genes and more strains and involve whole genomic sequencing.",
keywords = "House-keeping enzymes, MLST, Rodentibacter pneumotropicus, Rodents, Sequence type",
author = "Sadhana Adhikary and Magne Bisgaard and Ron Boot and Laurentiu Benga and Werner Nicklas and Henrik Christensen",
year = "2019",
doi = "10.1016/j.vetmic.2019.02.025",
language = "English",
volume = "231",
pages = "11--17",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus

AU - Adhikary, Sadhana

AU - Bisgaard, Magne

AU - Boot, Ron

AU - Benga, Laurentiu

AU - Nicklas, Werner

AU - Christensen, Henrik

PY - 2019

Y1 - 2019

N2 - The aim of the investigation was to develop a definitive typing system for Rodentibacter pneumotropicus. A total of 79 strains including the type strain of R. pneumotropicus, all associated with rodents were used to develop a multi-locus sequence typing scheme (MLST). Primers were designed for conserved regions of seven house-keeping genes (atpG, frdB, gdh, pgi, pmi, recA, zwf) and internal fragments of 399–839 bp were sequenced for all strains. The genes were also extracted in full length from whole genomic sequences of 14 strains of which 10 were sequenced in the current study. The number of alleles at the different loci ranged from 5 to 7 and a total of 20 allelic profiles or sequence types were recognized amongst the 79 strains. Analysis of the MLST data showed that some STs have been stable over many years probably circulating in the same colonies and probably transferred between colonies. We assume that this MLST scheme may provide a high level of resolution and might be an excellent tool for studying the population structure and epidemiology of R. pneumotropicus. Further development of the scheme is expected by including more genes and more strains and involve whole genomic sequencing.

AB - The aim of the investigation was to develop a definitive typing system for Rodentibacter pneumotropicus. A total of 79 strains including the type strain of R. pneumotropicus, all associated with rodents were used to develop a multi-locus sequence typing scheme (MLST). Primers were designed for conserved regions of seven house-keeping genes (atpG, frdB, gdh, pgi, pmi, recA, zwf) and internal fragments of 399–839 bp were sequenced for all strains. The genes were also extracted in full length from whole genomic sequences of 14 strains of which 10 were sequenced in the current study. The number of alleles at the different loci ranged from 5 to 7 and a total of 20 allelic profiles or sequence types were recognized amongst the 79 strains. Analysis of the MLST data showed that some STs have been stable over many years probably circulating in the same colonies and probably transferred between colonies. We assume that this MLST scheme may provide a high level of resolution and might be an excellent tool for studying the population structure and epidemiology of R. pneumotropicus. Further development of the scheme is expected by including more genes and more strains and involve whole genomic sequencing.

KW - House-keeping enzymes

KW - MLST

KW - Rodentibacter pneumotropicus

KW - Rodents

KW - Sequence type

U2 - 10.1016/j.vetmic.2019.02.025

DO - 10.1016/j.vetmic.2019.02.025

M3 - Journal article

VL - 231

SP - 11

EP - 17

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -

ID: 218182202