Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men

Research output: Contribution to journalJournal articleResearchpeer-review

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Cold-water immersion after training sessions : Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men. / Christiansen, Danny; Bishop, David John; Broatch, James R; Bangsbo, Jens; McKenna, Michael John; Murphy, Robyn M.

In: Journal of Applied Physiology, Vol. 125, No. 2, 2018, p. 429-444.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Christiansen, D, Bishop, DJ, Broatch, JR, Bangsbo, J, McKenna, MJ & Murphy, RM 2018, 'Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men', Journal of Applied Physiology, vol. 125, no. 2, pp. 429-444. https://doi.org/10.1152/japplphysiol.00259.2018

APA

Christiansen, D., Bishop, D. J., Broatch, J. R., Bangsbo, J., McKenna, M. J., & Murphy, R. M. (2018). Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men. Journal of Applied Physiology, 125(2), 429-444. https://doi.org/10.1152/japplphysiol.00259.2018

Vancouver

Christiansen D, Bishop DJ, Broatch JR, Bangsbo J, McKenna MJ, Murphy RM. Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men. Journal of Applied Physiology. 2018;125(2):429-444. https://doi.org/10.1152/japplphysiol.00259.2018

Author

Christiansen, Danny ; Bishop, David John ; Broatch, James R ; Bangsbo, Jens ; McKenna, Michael John ; Murphy, Robyn M. / Cold-water immersion after training sessions : Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men. In: Journal of Applied Physiology. 2018 ; Vol. 125, No. 2. pp. 429-444.

Bibtex

@article{c21bce32a2404137a8090e79207f889c,
title = "Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men",
abstract = "Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K+ transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally-active men (24{plus minus}6 y, 79.5{plus minus}10.8 kg, 44.6{plus minus}5.8 mL∙kg-1∙min-1) completed six weeks of sprint-interval cycling either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10{degree sign}C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na+,K+-ATPase isoforms (α1-3, β1-3) and FXYD1, and after recovery treatments (+0 h and +3 h) on the first day of training to measure mRNA content. Training increased (p<0.05) the abundance of α1 and β3 in both fiber types, β1 in type-II fibers, and decreased FXYD1 in type-I fibers, whereas α2 and α3 abundance was not altered by training (p>0.05). CWI after each session did not influence responses to training (p>0.05). However, α2 mRNA increased after the first session in COLD (+0 h, p<0.05), but not in CON (p>0.05). In both conditions, α1 and β3 mRNA increased (+3 h; p <0.05), β2 mRNA decreased (+3 h; p<0.05), whereas α3, β1, and FXYD1 mRNA remained unchanged (p>0.05) after the first session. In summary, Na+,K+-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which for most isoforms do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K+ regulation.",
keywords = "The Faculty of Science, Cold water immersion, Training, Human muscle, Fibre type, Ion transport, FXYD1, Na+,K+-ATPAse",
author = "Danny Christiansen and Bishop, {David John} and Broatch, {James R} and Jens Bangsbo and McKenna, {Michael John} and Murphy, {Robyn M}",
note = "CURIS 2018 NEXS 298",
year = "2018",
doi = "10.1152/japplphysiol.00259.2018",
language = "English",
volume = "125",
pages = "429--444",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "2",

}

RIS

TY - JOUR

T1 - Cold-water immersion after training sessions

T2 - Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men

AU - Christiansen, Danny

AU - Bishop, David John

AU - Broatch, James R

AU - Bangsbo, Jens

AU - McKenna, Michael John

AU - Murphy, Robyn M

N1 - CURIS 2018 NEXS 298

PY - 2018

Y1 - 2018

N2 - Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K+ transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally-active men (24{plus minus}6 y, 79.5{plus minus}10.8 kg, 44.6{plus minus}5.8 mL∙kg-1∙min-1) completed six weeks of sprint-interval cycling either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10{degree sign}C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na+,K+-ATPase isoforms (α1-3, β1-3) and FXYD1, and after recovery treatments (+0 h and +3 h) on the first day of training to measure mRNA content. Training increased (p<0.05) the abundance of α1 and β3 in both fiber types, β1 in type-II fibers, and decreased FXYD1 in type-I fibers, whereas α2 and α3 abundance was not altered by training (p>0.05). CWI after each session did not influence responses to training (p>0.05). However, α2 mRNA increased after the first session in COLD (+0 h, p<0.05), but not in CON (p>0.05). In both conditions, α1 and β3 mRNA increased (+3 h; p <0.05), β2 mRNA decreased (+3 h; p<0.05), whereas α3, β1, and FXYD1 mRNA remained unchanged (p>0.05) after the first session. In summary, Na+,K+-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which for most isoforms do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K+ regulation.

AB - Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K+ transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally-active men (24{plus minus}6 y, 79.5{plus minus}10.8 kg, 44.6{plus minus}5.8 mL∙kg-1∙min-1) completed six weeks of sprint-interval cycling either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10{degree sign}C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na+,K+-ATPase isoforms (α1-3, β1-3) and FXYD1, and after recovery treatments (+0 h and +3 h) on the first day of training to measure mRNA content. Training increased (p<0.05) the abundance of α1 and β3 in both fiber types, β1 in type-II fibers, and decreased FXYD1 in type-I fibers, whereas α2 and α3 abundance was not altered by training (p>0.05). CWI after each session did not influence responses to training (p>0.05). However, α2 mRNA increased after the first session in COLD (+0 h, p<0.05), but not in CON (p>0.05). In both conditions, α1 and β3 mRNA increased (+3 h; p <0.05), β2 mRNA decreased (+3 h; p<0.05), whereas α3, β1, and FXYD1 mRNA remained unchanged (p>0.05) after the first session. In summary, Na+,K+-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which for most isoforms do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K+ regulation.

KW - The Faculty of Science

KW - Cold water immersion

KW - Training

KW - Human muscle

KW - Fibre type

KW - Ion transport

KW - FXYD1

KW - Na+,K+-ATPAse

U2 - 10.1152/japplphysiol.00259.2018

DO - 10.1152/japplphysiol.00259.2018

M3 - Journal article

VL - 125

SP - 429

EP - 444

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -

ID: 196345320