Chromosomal mapping and mutational analysis of the coding region of the glycogen synthase kinase-3alpha and beta isoforms in patients with NIDDM

Research output: Contribution to journalJournal articleResearchpeer-review

L Hansen, K C Arden, S B Rasmussen, C S Viars, H Vestergaard, T Hansen, A M Møller, J R Woodgett, O Pedersen

Activation of glycogen synthesis in skeletal muscle in response to insulin results from the combined inactivation of glycogen synthase kinase-3 (GSK-3) and activation of the protein phosphatase-1, changing the ratio between the inactive phosphorylated state of the glycogen synthase to the active dephosphorylated state. In a search for genetic defects responsible for the decreased insulin stimulated glycogen synthesis seen in patients with non-insulin-dependent diabetes mellitus (NIDDM) and their glucose-tolerant first-degree relatives we have performed mutational analysis of the coding region of the 2 isoforms of GSK-3alpha and GSK-3beta in 72 NIDDM patients and 12 control subjects. No structural changes were detected apart from a few silent mutations. Mapping of the GSK-3alpha to chromosome 19q13.1-13.2 and the GSK-3beta to chromosome 3q13.3-q21 outside known genetic loci linked to NIDDM further makes it unlikely that these genes are involved in the pathogenesis of common forms of NIDDM.
Original languageEnglish
JournalDiabetologia
Volume40
Issue number8
Pages (from-to)940-6
Number of pages7
ISSN0012-186X
Publication statusPublished - Aug 1997

    Research areas

  • Alleles, Animals, Autoradiography, Base Sequence, Biopsy, Blotting, Southern, Calcium-Calmodulin-Dependent Protein Kinases, Chromosome Mapping, Cricetinae, DNA Mutational Analysis, DNA Primers, Diabetes Mellitus, Type 2, Glycogen Synthase Kinases, Humans, In Situ Hybridization, Fluorescence, Muscle, Skeletal, Mutation, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational

ID: 92192750