Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine

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C Orskov, T Buhl, L Rabenhøj, Hans Kofod, J J Holst

We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151-158, PG 151-160 and PG 126-159 (glucagon-like peptide-2 (GLP-2] in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126-159) and for PG 151-158, whereas the assay for PG 151-160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.
Original languageEnglish
JournalF E B S Letters
Volume247
Issue number2
Pages (from-to)193-6
Number of pages4
ISSN0014-5793
Publication statusPublished - 24 Apr 1989

    Research areas

  • Amino Acid Sequence, Animals, Carboxypeptidase B, Carboxypeptidases, Chromatography, Gel, Gastrointestinal Hormones, Glucagon-Like Peptide 2, Glucagon-Like Peptides, Humans, Intestine, Small, Molecular Sequence Data, Peptide Fragments, Peptides, Protein Precursors, Protein Processing, Post-Translational, Radioimmunoassay, Swine

ID: 45575003