Acute phase proteins as diagnostic markers in horses with colic
Research output: Contribution to journal › Journal article › Research › peer-review
- Pihl_2015_Acutephaseproteins as diagnostic markers in horses with colic_accepted_in press
Accepted author manuscript, 1 MB, PDF document
Objective – To investigate the diagnostic potential of the concentrations of acute-phase proteins serum amyloid A (SAA), haptoglobin (Hp), and ﬁbrinogen in blood and peritoneal ﬂuid (PF) for differentiating horses within ﬂammatory colic (entero-colitis and peritonitis) from those with surgical colic.
Design – Prospective observational multicenter study.
Setting – Two university referral hospitals.
Animals – Horses referred for severe acute abdominal pain to Hospital 1 (n = 148) or Hospital 2 (n = 78).
Intervention – Blood and PF samples collected at admission were used for acute-phase protein concentration measurement.
Measurements and Main Results – A multivariable logistic model including clinical parameters (lethargy, rectal temperature >38°C [100.4°F], normal rectal examination ﬁndings, and gastric reﬂux of 5–10 L) recorded atadmission was constructed from Hospital 1 data. The ability of the model to correctly differentiate inﬂammatory from surgical colic was 86% determined as area under the receiver operating characteristic curve. Adding blood parameters (WBC, PCV, total plasma protein, lactate, SAA, Hp, and ﬁbrinogen concentrations) to the logistic model based on clinical parameters revealed that only WBC and SAA and ﬁbrinogen concentrations improved the model. With SAA included in the model no additional blood parameters improved the model, and the ﬁnal model had an area under the curve of 90%. Addition of PF parameters (hemolysis, total protein concentration, WBC, SAA, or Hp concentrations) did not improve the model. When validated in Hospital 2 data, the models had good integrity and diagnostic performance.
Conclusions – Evaluation of SAA in serum improved the ability to differentiate horses with acute inﬂammatory colic requiring medical treatment from horses with colic requiring surgery, as it allowed an additional 4% of horses to be correctly classiﬁed into medical and surgical cases. Improved differentiation of these 2 groups of horses with colic may minimize the risk of unnecessary or delayed surgery.
|Journal||Journal of Veterinary Emergency and Critical Care|
|Number of pages||11|
|Publication status||Published - 2016|
- The Faculty of Health and Medical Sciences
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