Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation

Research output: Contribution to journalJournal articleResearchpeer-review

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Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation. / Stadler, Nadina; Stanley, Naomi; Heeneman, Sylvia; Vacata, Vladimir; Daemen, Mat J A P; Bannon, Paul G; Waltenberger, Johannes; Davies, Michael Jonathan.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 28, No. 5, 05.2008, p. 1024-30.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Stadler, N, Stanley, N, Heeneman, S, Vacata, V, Daemen, MJAP, Bannon, PG, Waltenberger, J & Davies, MJ 2008, 'Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 28, no. 5, pp. 1024-30. https://doi.org/10.1161/ATVBAHA.108.162735

APA

Stadler, N., Stanley, N., Heeneman, S., Vacata, V., Daemen, M. J. A. P., Bannon, P. G., ... Davies, M. J. (2008). Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation. Arteriosclerosis, Thrombosis, and Vascular Biology, 28(5), 1024-30. https://doi.org/10.1161/ATVBAHA.108.162735

Vancouver

Stadler N, Stanley N, Heeneman S, Vacata V, Daemen MJAP, Bannon PG et al. Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation. Arteriosclerosis, Thrombosis, and Vascular Biology. 2008 May;28(5):1024-30. https://doi.org/10.1161/ATVBAHA.108.162735

Author

Stadler, Nadina ; Stanley, Naomi ; Heeneman, Sylvia ; Vacata, Vladimir ; Daemen, Mat J A P ; Bannon, Paul G ; Waltenberger, Johannes ; Davies, Michael Jonathan. / Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2008 ; Vol. 28, No. 5. pp. 1024-30.

Bibtex

@article{53162bf6fb8540cc9211001dfaa1c0b0,
title = "Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation",
abstract = "OBJECTIVE: Oxidized lipids and proteins, as well as decreased antioxidant levels, have been detected in human atherosclerotic lesions, with oxidation catalyzed by iron and copper postulated to contribute to lesion development. Zinc has been postulated to displace iron from critical sites and thereby protect against damage. In this study, metal ion and protein oxidation levels were quantified in human carotid and abdominal artery specimens containing early-to-advanced lesions, to determine whether zinc concentrations correlate inversely with iron levels and protein oxidation.METHODS AND RESULTS: Metal ions were quantified by EPR and inductively coupled plasma mass spectroscopy. Native and oxidized protein side-chains were quantified by high-performance liquid chromatography. Elevated levels of zinc ( approximately 6-fold) were detected in advanced lesions compared to healthy tissue or early lesions. Zinc did not correlate negatively with iron or copper levels suggesting that zinc does not displace these metal ions. Highly significant positive correlations (P<0.005) were detected between zinc and calcium levels.CONCLUSIONS: Zinc did not correlate with low iron levels and reduced protein oxidation. These data indicate that zinc does not prevent protein oxidation in advanced lesions. The reported protective effect of zinc accumulation is proposed to be associated with lesion calcification.",
keywords = "Aged, Aged, 80 and over, Calcium, Carotid Artery Diseases, Carotid Stenosis, Copper, Humans, Iron, Middle Aged, Oxidation-Reduction, Proteins, Zinc",
author = "Nadina Stadler and Naomi Stanley and Sylvia Heeneman and Vladimir Vacata and Daemen, {Mat J A P} and Bannon, {Paul G} and Johannes Waltenberger and Davies, {Michael Jonathan}",
year = "2008",
month = "5",
doi = "10.1161/ATVBAHA.108.162735",
language = "English",
volume = "28",
pages = "1024--30",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams & Wilkins",
number = "5",

}

RIS

TY - JOUR

T1 - Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation

AU - Stadler, Nadina

AU - Stanley, Naomi

AU - Heeneman, Sylvia

AU - Vacata, Vladimir

AU - Daemen, Mat J A P

AU - Bannon, Paul G

AU - Waltenberger, Johannes

AU - Davies, Michael Jonathan

PY - 2008/5

Y1 - 2008/5

N2 - OBJECTIVE: Oxidized lipids and proteins, as well as decreased antioxidant levels, have been detected in human atherosclerotic lesions, with oxidation catalyzed by iron and copper postulated to contribute to lesion development. Zinc has been postulated to displace iron from critical sites and thereby protect against damage. In this study, metal ion and protein oxidation levels were quantified in human carotid and abdominal artery specimens containing early-to-advanced lesions, to determine whether zinc concentrations correlate inversely with iron levels and protein oxidation.METHODS AND RESULTS: Metal ions were quantified by EPR and inductively coupled plasma mass spectroscopy. Native and oxidized protein side-chains were quantified by high-performance liquid chromatography. Elevated levels of zinc ( approximately 6-fold) were detected in advanced lesions compared to healthy tissue or early lesions. Zinc did not correlate negatively with iron or copper levels suggesting that zinc does not displace these metal ions. Highly significant positive correlations (P<0.005) were detected between zinc and calcium levels.CONCLUSIONS: Zinc did not correlate with low iron levels and reduced protein oxidation. These data indicate that zinc does not prevent protein oxidation in advanced lesions. The reported protective effect of zinc accumulation is proposed to be associated with lesion calcification.

AB - OBJECTIVE: Oxidized lipids and proteins, as well as decreased antioxidant levels, have been detected in human atherosclerotic lesions, with oxidation catalyzed by iron and copper postulated to contribute to lesion development. Zinc has been postulated to displace iron from critical sites and thereby protect against damage. In this study, metal ion and protein oxidation levels were quantified in human carotid and abdominal artery specimens containing early-to-advanced lesions, to determine whether zinc concentrations correlate inversely with iron levels and protein oxidation.METHODS AND RESULTS: Metal ions were quantified by EPR and inductively coupled plasma mass spectroscopy. Native and oxidized protein side-chains were quantified by high-performance liquid chromatography. Elevated levels of zinc ( approximately 6-fold) were detected in advanced lesions compared to healthy tissue or early lesions. Zinc did not correlate negatively with iron or copper levels suggesting that zinc does not displace these metal ions. Highly significant positive correlations (P<0.005) were detected between zinc and calcium levels.CONCLUSIONS: Zinc did not correlate with low iron levels and reduced protein oxidation. These data indicate that zinc does not prevent protein oxidation in advanced lesions. The reported protective effect of zinc accumulation is proposed to be associated with lesion calcification.

KW - Aged

KW - Aged, 80 and over

KW - Calcium

KW - Carotid Artery Diseases

KW - Carotid Stenosis

KW - Copper

KW - Humans

KW - Iron

KW - Middle Aged

KW - Oxidation-Reduction

KW - Proteins

KW - Zinc

U2 - 10.1161/ATVBAHA.108.162735

DO - 10.1161/ATVBAHA.108.162735

M3 - Journal article

VL - 28

SP - 1024

EP - 1030

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 5

ER -

ID: 129670859