A randomised, controlled, crossover study of the effect of diet on angiopoietin-like protein 4 (ANGPTL4) through modification of the gut microbiome

Research output: Contribution to journalJournal articleResearchpeer-review

Documents

Trine Blædel, Jacob Bak Holm, Ulrik K. Sundekilde, Mette S. Schmedes, Anne Lundby Hess, Janne Kunchel Lorenzen, Karsten Kristiansen, Trine K. Dalsgaard, Arne Astrup, Lesli Hingstrup Larsen

Angiopoietin-like protein 4 (ANGPTL4) is a lipoprotein lipase inhibitor that is involved in lipid metabolism and angiogenesis. Animal studies have suggested
that the ANGPTL4 protein is modulated by the gut microbiota, possibly through increased concentrations of SCFA, such as C4, found in whole-fat milk or as a result of fermentation of inulin. This study investigated whether a standardised diet either high in fat content or supplemented with inulin powder would increase plasma ANGPTL4 in overweight men and whether this increase was mediated through a compositional change of the gut microbiota. The study had a crossover design with three arms, where participants were given a standardised isoenergetic diet supplemented with inulin powder, whole-fat milk or water (control). Plasma and urine samples were collected before and after each intervention period. Faecal samples and adipose tissue biopsies were collected after each intervention period. The study included twenty-one participants of whom eighteen completed the study. The dietary interventions did not change ANGPTL4 plasma concentration, nor was plasma ANGPTL4 associated with plasma lipids, TAG or NEFA concentration. The relative abundance of bifidobacteria following the inulin diet was higher, compared with the control diet. However, the changes in microbiota were not associated with plasma ANGPTL4 and the overall composition of the microbiota did not change between the dietary periods. Although weight was maintained throughout the dietary periods, weight was negatively associated with plasma ANGPTL4 concentration. In the adipose tissue, ANGPTL4 expression was correlated with leptin expression, but not with hypoxia-inducible factor 1α (HIF-1α) expression.
Original languageEnglish
Article numbere45
JournalJournal of Nutritional Science
Volume5
Number of pages10
DOIs
Publication statusPublished - 2016

    Research areas

  • The Faculty of Science - Angiopoietin-like protein 4, Lipid metabolism, Lipoprotein lipase, Obesity, Gut microbiome

Number of downloads are based on statistics from Google Scholar and www.ku.dk


No data available

ID: 174013961