64Cu loaded liposomes as positron emission tomography imaging agents

Research output: Contribution to journalJournal articleResearchpeer-review

Anncatrine Luisa Petersen, Tina Binderup, Palle Rasmussen, Jonas Rosager Henriksen, Dennis Ringkjøbing Elema, Andreas Kjær, Thomas Lars Andresen, Anncatrine Luisa Petersen, Tina Binderup, Palle Damkjær Rasmussen, Jonas Rosager Henriksen, Dennis Ringkjøbing Elema, Andreas Kjær, Thomas Lars Andresen

We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide ((64)Cu) using a new ionophore, 2-hydroxyquinoline, to carry (64)Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the (64)Cu-liposomes highly applicable as PET imaging agents. We show the utility of the (64)Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.
Original languageEnglish
JournalBiomaterials
Volume32
Issue number9
Pages (from-to)2334-41
Number of pages8
ISSN0142-9612
DOIs
Publication statusPublished - 2011

    Research areas

  • Animals, Calorimetry, Copper Radioisotopes, HT29 Cells, Humans, Ligands, Liposomes, Mice, Neoplasms, Particle Size, Positron-Emission Tomography, Static Electricity, Tissue Distribution

ID: 33790721