Stable markers of oxidant damage to proteins and their application in the study of human disease

Research output: Contribution to journalJournal articleResearchpeer-review

Michael Jonathan Davies, S Fu, H Wang, R T Dean

The mechanisms of formation and the nature of the altered amino acid side chains formed on proteins subjected to oxidant attack are reviewed. The use of stable products of protein side chain oxidation as potential markers for assessing oxidative damage in vivo in humans is discussed. The methods developed in the authors laboratories are outlined, and the advantages and disadvantages of these techniques compared with other methodologies for assessing oxidative damage to proteins and other macromolecules. Evidence is presented to show that protein oxidation products are sensitive markers of oxidative damage, that the pattern of products detected may yield information as to the nature of the original oxidative insult, and that the levels of oxidized side-chains can, in certain circumstances, be much higher than those of other markers of oxidation such as lipid hydroperoxides.

Original languageEnglish
JournalFree Radical Biology & Medicine
Volume27
Issue number11-12
Pages (from-to)1151-63
Number of pages13
ISSN0891-5849
Publication statusPublished - Dec 1999

    Research areas

  • Amino Acids, Animals, Biological Markers, Disease, Humans, Oxidants, Oxidation-Reduction, Oxidative Stress, Proteins

ID: 138282023