Formation of long-lived radicals on proteins by radical transfer from heme enzymes--a common process?

Research output: Contribution to journalJournal articleResearchpeer-review

H Ostdal, H J Andersen, Michael Jonathan Davies

Incubation of Fe(III)myoglobin (Fe(III)Mb) with H2O2 in the presence of bovine serum albumin (BSA) has been shown previously to give albumin-derived radicals as a result of radical transfer from myoglobin to BSA. In this study the occurrence of similar processes with peroxidases has been investigated using horseradish peroxidase (HRP)/H2O2, in the presence and absence of added tyrosine. Incubation of HRP with H2O2 and bovine or human serum albumins, in the presence and absence of tyrosine, gave long-lived albumin-derived radicals as detected by EPR spectroscopy. Evidence has been obtained for these albumin radicals being located on buried tyrosine residues on the basis of blocking experiments. The effect of protein conformation on radical transfer has been investigated using partial proteolytic digestion prior to protein oxidation. With HRP/H2O2/BSA and Fe(III)Mb/H2O2/BSA increased radical concentrations were observed after limited digestion, although this effect was less marked with the HRP/H2O2/BSA system than with Fe(III)Mb/H2O2/BSA, consistent with different modes of radical transfer. More extensive digestion of BSA decreased the radical concentration to levels below those detected with native albumin, indicating that the tertiary structure of the target protein plays an important role in determining the rate of radical transfer and/or the stability of the resultant species. These results are consistent with a mechanism for the HRP/H2O2/no free tyrosine system involving radical transfer to the albumin via the heme edge of the peroxidase. In contrast, albumin radical formation by the HRP/H2O2/free tyrosine system was only marginally affected by proteolysis, consistent with free tyrosine phenoxyl radicals being the mediators of radical transfer, without significant protein-protein interaction. These protein-to-protein radical transfer reactions may have important consequences for understanding protein oxidation in biological systems.

Original languageEnglish
JournalArchives of Biochemistry and Biophysics
Volume362
Issue number1
Pages (from-to)105-12
Number of pages8
ISSN0003-9861
DOIs
Publication statusPublished - 1 Feb 1999

    Research areas

  • Animals, Cattle, Electron Spin Resonance Spectroscopy, Free Radicals, Hemeproteins, Horseradish Peroxidase, Horses, Humans, Hydrogen Peroxide, Metmyoglobin, Oxidation-Reduction, Pronase, Protein Structure, Secondary, Protein Structure, Tertiary, Serum Albumin, Bovine, Tyrosine

ID: 138282907