Endoproteolytic activities in pea roots inoculated with the arbuscular mycorrhizal fungus Glomus mosseae and/orAphanomyces euteiches in relation to bioprotection
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Endoproteolytic activities in pea roots inoculated with the arbuscular mycorrhizal fungus Glomus mosseae and/orAphanomyces euteiches in relation to bioprotection. / Slezack, S; DUMAS-GAUDOT, E; Rosendahl, Søren; Kjøller, Rasmus; Paynot, M; Negrel, J; Gianinazzi, S.
In: New Phytologist, Vol. 142, No. 3, 1999, p. 517-529.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Endoproteolytic activities in pea roots inoculated with the arbuscular mycorrhizal fungus Glomus mosseae and/orAphanomyces euteiches in relation to bioprotection
AU - Slezack, S
AU - DUMAS-GAUDOT, E
AU - Rosendahl, Søren
AU - Kjøller, Rasmus
AU - Paynot, M
AU - Negrel, J
AU - Gianinazzi, S
N1 - KEYWORDS endoproteolytic activities • arbuscular mycorrhizal fungi • Pisum sativum • Aphanomyces euteiches • bioprotection
PY - 1999
Y1 - 1999
N2 - Arbuscular mycorrhizal (AM) symbioses are known to play a role in increased resistance of plants against soilborne pathogens. Mechanisms involved in this phenomenon are not yet well understood. This work investigates possible roles of endoproteolytic activities in bioprotection of Pisum sativum roots by Glomus mosseae against Aphanomyces euteiches. First, it is demonstrated that bioprotection occurs only in pre-mycorrhizal plants. Second, endoproteolytic activities were analysed qualitatively and quantitatively during AM symbiosis, in plants infected with either zoospores or mycelium of A. euteiches, and in mycorrhizal plants infected with the pathogen. In mycorrhizal symbiosis a progressive increase in endoproteolytic activities was observed following root colonization by G. mosseae. By contrast, in roots inoculated with A. euteiches, a drastic increase in endoproteolytic activities was observed which was correlated with the amount of pathogen occurring in roots. Qualitative differences were seen among the endoproteolytic activities detected in roots inoculated with zoospores or mycelium. The constitutive as well as mycorrhizal and pathogen-induced activities were further characterized as 'trypsin-like' serine endoproteases. Interestingly, in a situation of bioprotection, only low levels of the activities normally associated with the infection by A. euteiches were detected, suggesting that the synthesis of these proteins is directly linked to the growth or virulence of the pathogen.
AB - Arbuscular mycorrhizal (AM) symbioses are known to play a role in increased resistance of plants against soilborne pathogens. Mechanisms involved in this phenomenon are not yet well understood. This work investigates possible roles of endoproteolytic activities in bioprotection of Pisum sativum roots by Glomus mosseae against Aphanomyces euteiches. First, it is demonstrated that bioprotection occurs only in pre-mycorrhizal plants. Second, endoproteolytic activities were analysed qualitatively and quantitatively during AM symbiosis, in plants infected with either zoospores or mycelium of A. euteiches, and in mycorrhizal plants infected with the pathogen. In mycorrhizal symbiosis a progressive increase in endoproteolytic activities was observed following root colonization by G. mosseae. By contrast, in roots inoculated with A. euteiches, a drastic increase in endoproteolytic activities was observed which was correlated with the amount of pathogen occurring in roots. Qualitative differences were seen among the endoproteolytic activities detected in roots inoculated with zoospores or mycelium. The constitutive as well as mycorrhizal and pathogen-induced activities were further characterized as 'trypsin-like' serine endoproteases. Interestingly, in a situation of bioprotection, only low levels of the activities normally associated with the infection by A. euteiches were detected, suggesting that the synthesis of these proteins is directly linked to the growth or virulence of the pathogen.
U2 - 10.1046/j.1469-8137.1999.00421.x
DO - 10.1046/j.1469-8137.1999.00421.x
M3 - Journal article
VL - 142
SP - 517
EP - 529
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 3
ER -
ID: 10729141