Effect of exposure of human monocyte-derived macrophages to high, versus normal, glucose on subsequent lipid accumulation from glycated and acetylated low-density lipoproteins

Research output: Contribution to journalJournal articleResearchpeer-review

Fatemeh Moheimani, Joanne T M Tan, Bronwyn E Brown, Alison K Heather, David M van Reyk, Michael Jonathan Davies

During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-like oxidised LDL receptor-1 (LOX-1) and class AI and AII (SR-AI, SR-AII) and class B (SR-BI, CD36) scavenger receptors. Here we examined the hypothesis that hyperglycaemia may modulate receptor expression and hence lipid accumulation in macrophages. Human monocytes were matured into macrophages in 30 versus 5 mM glucose and receptor expression and lipid accumulation quantified. High glucose elevated LOX1 mRNA, but decreased SR-AI, SR-BI, LDLR, and CD36 mRNA. SR-BI and CD36 protein levels were decreased. Normo- and hyperglycaemic cells accumulated cholesteryl esters from modified LDL to a greater extent than control LDL, but total and individual cholesteryl ester accumulation was not affected by glucose levels. It is concluded that, whilst macrophage scavenger receptor mRNA and protein levels can be modulated by high glucose, these are not key factors in lipid accumulation by human macrophages under the conditions examined.

Original languageEnglish
JournalJournal of Diabetes Research
Volume2011
Pages (from-to)851280
ISSN2314-6745
DOIs
Publication statusPublished - 2011
Externally publishedYes

    Research areas

  • Antigens, CD36, Atherosclerosis, Base Sequence, Cell Differentiation, Cells, Cultured, Cholesterol Esters, DNA Primers, Glucose, Humans, Lipid Metabolism, Lipoproteins, LDL, Macrophages, RNA, Messenger, Receptors, LDL, Scavenger Receptors, Class A, Scavenger Receptors, Class B, Scavenger Receptors, Class E

ID: 129669420