Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts

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Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts. / Rahmanto, Aldwin Suryo; Morgan, Philip E; Hawkins, Clare Louise; Davies, Michael Jonathan.

In: Free Radical Biology & Medicine, Vol. 49, No. 10, 30.11.2010, p. 1505-15.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rahmanto, AS, Morgan, PE, Hawkins, CL & Davies, MJ 2010, 'Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts', Free Radical Biology & Medicine, vol. 49, no. 10, pp. 1505-15. https://doi.org/10.1016/j.freeradbiomed.2010.08.006

APA

Rahmanto, A. S., Morgan, P. E., Hawkins, C. L., & Davies, M. J. (2010). Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts. Free Radical Biology & Medicine, 49(10), 1505-15. https://doi.org/10.1016/j.freeradbiomed.2010.08.006

Vancouver

Rahmanto AS, Morgan PE, Hawkins CL, Davies MJ. Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts. Free Radical Biology & Medicine. 2010 Nov 30;49(10):1505-15. https://doi.org/10.1016/j.freeradbiomed.2010.08.006

Author

Rahmanto, Aldwin Suryo ; Morgan, Philip E ; Hawkins, Clare Louise ; Davies, Michael Jonathan. / Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts. In: Free Radical Biology & Medicine. 2010 ; Vol. 49, No. 10. pp. 1505-15.

Bibtex

@article{71fc091ce3b64336ba747a59b6b8b3d6,
title = "Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts",
abstract = "Reaction of radicals and singlet oxygen ((1)O(2)) with proteins results in both direct damage and the formation of long-lived reactive hydroperoxides. Elevated levels of protein hydroperoxide-derived products have been detected in multiple human pathologies, suggesting that these secondary oxidants contribute to tissue damage. Previous studies have provided evidence for protein hydroperoxide-mediated inhibition of thiol-dependent enzymes and modulation of signaling processes in isolated systems. In this study (1)O(2) and hydroperoxides have been generated in J774A.1 macrophage-like cells using visible light and the photosensitizer rose bengal, with the consequences of oxidant formation examined both immediately and after subsequent (dark-phase) incubation. Significant losses of GSH (≤50{\%}), total thiols (≤20{\%}), and activity of thiol-dependent proteins (GAPDH, thioredoxin, protein tyrosine phosphatases, creatine kinase, and cathepsins B and L; 10-50{\%} inhibition) were detected after 1 or 2 min photo-oxidation. Non-thiol-dependent enzymes were not affected. In contrast, NADPH levels increased, together with the activity of glutathione reductase, glutathione peroxidase, and thioredoxin reductase; these increases may be components of a rapid global cytoprotective cellular response to stress. Neither oxidized thioredoxin nor radical-mediated protein oxidation products were detected at significant levels. Further decreases in thiol levels and enzyme activity occurred during dark-phase incubation, with this accompanied by decreased cell viability. These secondary events are ascribed to the reactions of long-lived hydroperoxides, generated by (1)O(2)-mediated reactions. Overall, this study provides novel insights into early cellular responses to photo-oxidative damage and indicates that long-lived hydroperoxides can play a significant role in cellular damage.",
keywords = "Animals, Antioxidants, Cell Survival, Cells, Cultured, Glutathione, Glutathione Peroxidase, Glutathione Reductase, Humans, Hydrogen Peroxide, Macrophages, Mice, Oxidants, Photochemical Processes, Proteins, Rose Bengal, Singlet Oxygen, Sulfhydryl Compounds, Thioredoxins",
author = "Rahmanto, {Aldwin Suryo} and Morgan, {Philip E} and Hawkins, {Clare Louise} and Davies, {Michael Jonathan}",
note = "Copyright {\circledC} 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = "11",
day = "30",
doi = "10.1016/j.freeradbiomed.2010.08.006",
language = "English",
volume = "49",
pages = "1505--15",
journal = "Free Radical Biology & Medicine",
issn = "0891-5849",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts

AU - Rahmanto, Aldwin Suryo

AU - Morgan, Philip E

AU - Hawkins, Clare Louise

AU - Davies, Michael Jonathan

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2010/11/30

Y1 - 2010/11/30

N2 - Reaction of radicals and singlet oxygen ((1)O(2)) with proteins results in both direct damage and the formation of long-lived reactive hydroperoxides. Elevated levels of protein hydroperoxide-derived products have been detected in multiple human pathologies, suggesting that these secondary oxidants contribute to tissue damage. Previous studies have provided evidence for protein hydroperoxide-mediated inhibition of thiol-dependent enzymes and modulation of signaling processes in isolated systems. In this study (1)O(2) and hydroperoxides have been generated in J774A.1 macrophage-like cells using visible light and the photosensitizer rose bengal, with the consequences of oxidant formation examined both immediately and after subsequent (dark-phase) incubation. Significant losses of GSH (≤50%), total thiols (≤20%), and activity of thiol-dependent proteins (GAPDH, thioredoxin, protein tyrosine phosphatases, creatine kinase, and cathepsins B and L; 10-50% inhibition) were detected after 1 or 2 min photo-oxidation. Non-thiol-dependent enzymes were not affected. In contrast, NADPH levels increased, together with the activity of glutathione reductase, glutathione peroxidase, and thioredoxin reductase; these increases may be components of a rapid global cytoprotective cellular response to stress. Neither oxidized thioredoxin nor radical-mediated protein oxidation products were detected at significant levels. Further decreases in thiol levels and enzyme activity occurred during dark-phase incubation, with this accompanied by decreased cell viability. These secondary events are ascribed to the reactions of long-lived hydroperoxides, generated by (1)O(2)-mediated reactions. Overall, this study provides novel insights into early cellular responses to photo-oxidative damage and indicates that long-lived hydroperoxides can play a significant role in cellular damage.

AB - Reaction of radicals and singlet oxygen ((1)O(2)) with proteins results in both direct damage and the formation of long-lived reactive hydroperoxides. Elevated levels of protein hydroperoxide-derived products have been detected in multiple human pathologies, suggesting that these secondary oxidants contribute to tissue damage. Previous studies have provided evidence for protein hydroperoxide-mediated inhibition of thiol-dependent enzymes and modulation of signaling processes in isolated systems. In this study (1)O(2) and hydroperoxides have been generated in J774A.1 macrophage-like cells using visible light and the photosensitizer rose bengal, with the consequences of oxidant formation examined both immediately and after subsequent (dark-phase) incubation. Significant losses of GSH (≤50%), total thiols (≤20%), and activity of thiol-dependent proteins (GAPDH, thioredoxin, protein tyrosine phosphatases, creatine kinase, and cathepsins B and L; 10-50% inhibition) were detected after 1 or 2 min photo-oxidation. Non-thiol-dependent enzymes were not affected. In contrast, NADPH levels increased, together with the activity of glutathione reductase, glutathione peroxidase, and thioredoxin reductase; these increases may be components of a rapid global cytoprotective cellular response to stress. Neither oxidized thioredoxin nor radical-mediated protein oxidation products were detected at significant levels. Further decreases in thiol levels and enzyme activity occurred during dark-phase incubation, with this accompanied by decreased cell viability. These secondary events are ascribed to the reactions of long-lived hydroperoxides, generated by (1)O(2)-mediated reactions. Overall, this study provides novel insights into early cellular responses to photo-oxidative damage and indicates that long-lived hydroperoxides can play a significant role in cellular damage.

KW - Animals

KW - Antioxidants

KW - Cell Survival

KW - Cells, Cultured

KW - Glutathione

KW - Glutathione Peroxidase

KW - Glutathione Reductase

KW - Humans

KW - Hydrogen Peroxide

KW - Macrophages

KW - Mice

KW - Oxidants

KW - Photochemical Processes

KW - Proteins

KW - Rose Bengal

KW - Singlet Oxygen

KW - Sulfhydryl Compounds

KW - Thioredoxins

U2 - 10.1016/j.freeradbiomed.2010.08.006

DO - 10.1016/j.freeradbiomed.2010.08.006

M3 - Journal article

VL - 49

SP - 1505

EP - 1515

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 10

ER -

ID: 129669952