Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow

Research output: Contribution to journalJournal articleResearchpeer-review

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Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow. / Jensen, Elisa Pouline; Poulsen, Steen Seier; Kissow, Hannelouise; Holstein-Rathlou, Niels-Henrik; Deacon, Carolyn F.; Jensen, Boye L.; Holst, Jens J.; Sorensen, Charlotte M.

In: A J P: Renal Physiology (Online), Vol. 308, No. 8, 15.04.2015, p. F867-F877.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jensen, EP, Poulsen, SS, Kissow, H, Holstein-Rathlou, N-H, Deacon, CF, Jensen, BL, Holst, JJ & Sorensen, CM 2015, 'Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow', A J P: Renal Physiology (Online), vol. 308, no. 8, pp. F867-F877. https://doi.org/10.1152/ajprenal.00527.2014

APA

Jensen, E. P., Poulsen, S. S., Kissow, H., Holstein-Rathlou, N-H., Deacon, C. F., Jensen, B. L., ... Sorensen, C. M. (2015). Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow. A J P: Renal Physiology (Online), 308(8), F867-F877. https://doi.org/10.1152/ajprenal.00527.2014

Vancouver

Jensen EP, Poulsen SS, Kissow H, Holstein-Rathlou N-H, Deacon CF, Jensen BL et al. Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow. A J P: Renal Physiology (Online). 2015 Apr 15;308(8):F867-F877. https://doi.org/10.1152/ajprenal.00527.2014

Author

Jensen, Elisa Pouline ; Poulsen, Steen Seier ; Kissow, Hannelouise ; Holstein-Rathlou, Niels-Henrik ; Deacon, Carolyn F. ; Jensen, Boye L. ; Holst, Jens J. ; Sorensen, Charlotte M. / Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow. In: A J P: Renal Physiology (Online). 2015 ; Vol. 308, No. 8. pp. F867-F877.

Bibtex

@article{777a0274b86d461cb291137bf2b092b8,
title = "Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow",
abstract = "Glucagon-like peptide-1 (GLP-1) has a range of extra-pancreatic effects, including renal. The mechanisms are poorly understood, but GLP-1 receptors have been identified in the kidney. However, the exact cellular localization of the renal receptors is poorly described. The aim of this study was to localize renal GLP-1 receptors and describe GLP-1 mediated effects on the renal vasculature. We hypothesized that renal GLP-1 receptors are located in the renal microcirculation and activation of these affects renal autoregulation and increases renal blood flow. In vivo autoradiography using 125I-GLP-1, 125I-exendin-4 (GLP-1 analog) and 125I-exendin 9-39 (GLP-1 receptor antagonist) was performed in rodents to localize specific GLP-1 receptor binding. GLP-1 mediated effects on blood pressure (BP), renal blood flow (RBF), heart rate (HR), renin secretion, urinary flow rate and Na+ and K+ excretion were investigated in anesthetized rats. Effects of GLP-1 on afferent arterioles were investigated in isolated mouse kidneys. Specific binding of 125I-GLP-1, 125I-exendin-4 and 125I-exendin 9-39 was observed in the renal vasculature including afferent arterioles. Infusion of GLP-1 increased BP, RBF and urinary flow rate significantly in rats. HR and plasma renin concentrations were unchanged. Exendin 9-39 inhibited the increase in RBF. In isolated murine kidneys GLP-1 and exendin-4 significantly reduced the autoregulatory response of the afferent arteriole in response to stepwise increases in pressure. We conclude that GLP-1 receptors are located in the renal vasculature including afferent arterioles. Activation of these receptors reduces the autoregulatory response of afferent arterioles to acute pressure increases and increases renal blood flow in normotensive rats.",
author = "Jensen, {Elisa Pouline} and Poulsen, {Steen Seier} and Hannelouise Kissow and Niels-Henrik Holstein-Rathlou and Deacon, {Carolyn F.} and Jensen, {Boye L.} and Holst, {Jens J.} and Sorensen, {Charlotte M.}",
note = "Copyright {\circledC} 2014, American Journal of Physiology - Renal Physiology.",
year = "2015",
month = "4",
day = "15",
doi = "10.1152/ajprenal.00527.2014",
language = "English",
volume = "308",
pages = "F867--F877",
journal = "A J P: Renal Physiology (Online)",
issn = "1522-1466",
publisher = "American Physiological Society",
number = "8",

}

RIS

TY - JOUR

T1 - Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow

AU - Jensen, Elisa Pouline

AU - Poulsen, Steen Seier

AU - Kissow, Hannelouise

AU - Holstein-Rathlou, Niels-Henrik

AU - Deacon, Carolyn F.

AU - Jensen, Boye L.

AU - Holst, Jens J.

AU - Sorensen, Charlotte M.

N1 - Copyright © 2014, American Journal of Physiology - Renal Physiology.

PY - 2015/4/15

Y1 - 2015/4/15

N2 - Glucagon-like peptide-1 (GLP-1) has a range of extra-pancreatic effects, including renal. The mechanisms are poorly understood, but GLP-1 receptors have been identified in the kidney. However, the exact cellular localization of the renal receptors is poorly described. The aim of this study was to localize renal GLP-1 receptors and describe GLP-1 mediated effects on the renal vasculature. We hypothesized that renal GLP-1 receptors are located in the renal microcirculation and activation of these affects renal autoregulation and increases renal blood flow. In vivo autoradiography using 125I-GLP-1, 125I-exendin-4 (GLP-1 analog) and 125I-exendin 9-39 (GLP-1 receptor antagonist) was performed in rodents to localize specific GLP-1 receptor binding. GLP-1 mediated effects on blood pressure (BP), renal blood flow (RBF), heart rate (HR), renin secretion, urinary flow rate and Na+ and K+ excretion were investigated in anesthetized rats. Effects of GLP-1 on afferent arterioles were investigated in isolated mouse kidneys. Specific binding of 125I-GLP-1, 125I-exendin-4 and 125I-exendin 9-39 was observed in the renal vasculature including afferent arterioles. Infusion of GLP-1 increased BP, RBF and urinary flow rate significantly in rats. HR and plasma renin concentrations were unchanged. Exendin 9-39 inhibited the increase in RBF. In isolated murine kidneys GLP-1 and exendin-4 significantly reduced the autoregulatory response of the afferent arteriole in response to stepwise increases in pressure. We conclude that GLP-1 receptors are located in the renal vasculature including afferent arterioles. Activation of these receptors reduces the autoregulatory response of afferent arterioles to acute pressure increases and increases renal blood flow in normotensive rats.

AB - Glucagon-like peptide-1 (GLP-1) has a range of extra-pancreatic effects, including renal. The mechanisms are poorly understood, but GLP-1 receptors have been identified in the kidney. However, the exact cellular localization of the renal receptors is poorly described. The aim of this study was to localize renal GLP-1 receptors and describe GLP-1 mediated effects on the renal vasculature. We hypothesized that renal GLP-1 receptors are located in the renal microcirculation and activation of these affects renal autoregulation and increases renal blood flow. In vivo autoradiography using 125I-GLP-1, 125I-exendin-4 (GLP-1 analog) and 125I-exendin 9-39 (GLP-1 receptor antagonist) was performed in rodents to localize specific GLP-1 receptor binding. GLP-1 mediated effects on blood pressure (BP), renal blood flow (RBF), heart rate (HR), renin secretion, urinary flow rate and Na+ and K+ excretion were investigated in anesthetized rats. Effects of GLP-1 on afferent arterioles were investigated in isolated mouse kidneys. Specific binding of 125I-GLP-1, 125I-exendin-4 and 125I-exendin 9-39 was observed in the renal vasculature including afferent arterioles. Infusion of GLP-1 increased BP, RBF and urinary flow rate significantly in rats. HR and plasma renin concentrations were unchanged. Exendin 9-39 inhibited the increase in RBF. In isolated murine kidneys GLP-1 and exendin-4 significantly reduced the autoregulatory response of the afferent arteriole in response to stepwise increases in pressure. We conclude that GLP-1 receptors are located in the renal vasculature including afferent arterioles. Activation of these receptors reduces the autoregulatory response of afferent arterioles to acute pressure increases and increases renal blood flow in normotensive rats.

U2 - 10.1152/ajprenal.00527.2014

DO - 10.1152/ajprenal.00527.2014

M3 - Journal article

VL - 308

SP - F867-F877

JO - A J P: Renal Physiology (Online)

JF - A J P: Renal Physiology (Online)

SN - 1522-1466

IS - 8

ER -

ID: 131197639