Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis
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Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis. / Christoffersen, Mette; Woodward, Elizabeth; Bojesen, Anders Miki; Jacobsen, Stine; Petersen, Morten Rønn; Troedsson, Mats H. T.; Lehn-Jensen, Henrik.
In: B M C Veterinary Research, Vol. 8, No. 41, 2012.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis
AU - Christoffersen, Mette
AU - Woodward, Elizabeth
AU - Bojesen, Anders Miki
AU - Jacobsen, Stine
AU - Petersen, Morten Rønn
AU - Troedsson, Mats H. T.
AU - Lehn-Jensen, Henrik
PY - 2012
Y1 - 2012
N2 - The objective of the study was to evaluate the gene expression of inflammatory cytokines (interleukin [IL]-1ß, IL-6, IL-8, IL-10, tumor necrosis factor [TNF]-a, IL-1 receptor antagonist [ra] and serum amyloid A (SAA) in endometrial tissue and circulating leukocytes in response to uterine inoculation of 105 colony forming units (CFU) Escherichia coli in mares. Before inoculation, mares were classified as resistant or susceptible to persistent endometritis based on their uterine inflammatory response to infusion of 109 killed spermatozoa and histological assessment of the endometrial quality. Endometrial biopsies were obtained 3, 12, 24 and 72 hours (h) after bacterial inoculation and blood samples were obtained during the 7 day period post bacterial inoculation. Expression levels of cytokines and SAA were determined by quantitative real-time reverse transcriptase PCR (qRT-PCR).
AB - The objective of the study was to evaluate the gene expression of inflammatory cytokines (interleukin [IL]-1ß, IL-6, IL-8, IL-10, tumor necrosis factor [TNF]-a, IL-1 receptor antagonist [ra] and serum amyloid A (SAA) in endometrial tissue and circulating leukocytes in response to uterine inoculation of 105 colony forming units (CFU) Escherichia coli in mares. Before inoculation, mares were classified as resistant or susceptible to persistent endometritis based on their uterine inflammatory response to infusion of 109 killed spermatozoa and histological assessment of the endometrial quality. Endometrial biopsies were obtained 3, 12, 24 and 72 hours (h) after bacterial inoculation and blood samples were obtained during the 7 day period post bacterial inoculation. Expression levels of cytokines and SAA were determined by quantitative real-time reverse transcriptase PCR (qRT-PCR).
KW - Animals
KW - Biopsy
KW - Cytokines
KW - Endometritis
KW - Female
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Horse Diseases
KW - Horses
KW - Inflammation
KW - Least-Squares Analysis
KW - RNA, Messenger
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Infectious endometritis,
KW - Susceptibility
KW - Cytokines
KW - Serum amyloid A
U2 - 10.1186/1746-6148-8-41
DO - 10.1186/1746-6148-8-41
M3 - Journal article
C2 - 22458733
VL - 8
JO - B M C Veterinary Research
JF - B M C Veterinary Research
SN - 1746-6148
IS - 41
ER -
ID: 44663534