Effect of buffers and pH in antenna sensitized Eu(III) luminescence
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Effect of buffers and pH in antenna sensitized Eu(III) luminescence. / Nielsen, Lea Gundorff; Sørensen, Thomas Just.
In: Methods and Applications in Fluorescence, Vol. 11, No. 1, 015005, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of buffers and pH in antenna sensitized Eu(III) luminescence
AU - Nielsen, Lea Gundorff
AU - Sørensen, Thomas Just
N1 - Funding Information: We thank the Carlsbergfoundation, Villum Fonden, Novo Nordisk Fonden, Eliteforsk, and the University of Copenhagen for generous support. We thank Ruifang Su for assistance in the preparation of the manuscript. Publisher Copyright: © 2023 IOP Publishing Ltd.
PY - 2023
Y1 - 2023
N2 - The photophysics of a europium(III) complex of 1,4,7,10-tetraazacycododecane-1,4,7-triacetic acid-10-(2-methylene)-1-azathioxanthone was investigated in three buffer systems and at three pH values. The buffers—phosphate buffered saline (PBS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and universal buffer (UB)—had no effect on the europium luminescence, but a lower overall emission intensity was determined in HEPES. It was found that this was due to quenching of the 1-azathioxanthone first excited singlet state by HEPES. The effect of pH on the photophysics of the complex was found to be minimal, and protonation of the pyridine nitrogen was found to be irrelevant. Even so, pH was shown to change the intensity ratio between 1-azathioxanthone fluorescence and europium luminescence. It was concluded that the full photophysics of a potential molecular probe should be investigated to achieve the best possible results in any application.
AB - The photophysics of a europium(III) complex of 1,4,7,10-tetraazacycododecane-1,4,7-triacetic acid-10-(2-methylene)-1-azathioxanthone was investigated in three buffer systems and at three pH values. The buffers—phosphate buffered saline (PBS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and universal buffer (UB)—had no effect on the europium luminescence, but a lower overall emission intensity was determined in HEPES. It was found that this was due to quenching of the 1-azathioxanthone first excited singlet state by HEPES. The effect of pH on the photophysics of the complex was found to be minimal, and protonation of the pyridine nitrogen was found to be irrelevant. Even so, pH was shown to change the intensity ratio between 1-azathioxanthone fluorescence and europium luminescence. It was concluded that the full photophysics of a potential molecular probe should be investigated to achieve the best possible results in any application.
KW - buffer effects
KW - lanthanide luminescence
KW - pH effects
KW - photophysics
U2 - 10.1088/2050-6120/acb63a
DO - 10.1088/2050-6120/acb63a
M3 - Journal article
C2 - 36696692
AN - SCOPUS:85147720737
VL - 11
JO - Methods and Applications in Fluorescence
JF - Methods and Applications in Fluorescence
SN - 2050-6120
IS - 1
M1 - 015005
ER -
ID: 338437058