The miRNA Plasma Signature in Response to Acute Aerobic Exercise and Endurance Training
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The miRNA Plasma Signature in Response to Acute Aerobic Exercise and Endurance Training. / Nielsen, Søren; Åkerström, Thorbjörn; Rinnov, Anders; Yfanti, Christina; Schéele, Camilla Charlotte; Pedersen, Bente Klarlund; Laye, Matthew James.
In: P L o S One, Vol. 9, No. 2, e87308, 2014.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The miRNA Plasma Signature in Response to Acute Aerobic Exercise and Endurance Training
AU - Nielsen, Søren
AU - Åkerström, Thorbjörn
AU - Rinnov, Anders
AU - Yfanti, Christina
AU - Schéele, Camilla Charlotte
AU - Pedersen, Bente Klarlund
AU - Laye, Matthew James
N1 - CURIS 2014 NEXS 092
PY - 2014
Y1 - 2014
N2 - MiRNAs are potent intracellular posttranscriptional regulators and are also selectively secreted into the circulation in a cell-specific fashion. Global changes in miRNA expression in skeletal muscle in response to endurance exercise training have been reported. Therefore, our aim was to establish the miRNA signature in human plasma in response to acute exercise and chronic endurance training by utilizing a novel methodological approach. RNA was isolated from human plasma collected from young healthy men before and after an acute endurance exercise bout and following 12 weeks of endurance training. Global miRNA (742 miRNAs) measurements were performed as a screening to identify detectable miRNAs in plasma. Using customized qPCR panels we quantified the expression levels of miRNAs detected in the screening procedure (188 miRNAs). We demonstrate a dynamic regulation of circulating miRNA (ci-miRNA) levels following 0 hour (miR-106a, miR-221, miR-30b, miR-151-5p, let-7i, miR-146, miR-652 and miR-151-3p), 1 hour (miR-338-3p, miR-330-3p, miR-223, miR-139-5p and miR-143) and 3 hours (miR-1) after an acute exercise bout (P
AB - MiRNAs are potent intracellular posttranscriptional regulators and are also selectively secreted into the circulation in a cell-specific fashion. Global changes in miRNA expression in skeletal muscle in response to endurance exercise training have been reported. Therefore, our aim was to establish the miRNA signature in human plasma in response to acute exercise and chronic endurance training by utilizing a novel methodological approach. RNA was isolated from human plasma collected from young healthy men before and after an acute endurance exercise bout and following 12 weeks of endurance training. Global miRNA (742 miRNAs) measurements were performed as a screening to identify detectable miRNAs in plasma. Using customized qPCR panels we quantified the expression levels of miRNAs detected in the screening procedure (188 miRNAs). We demonstrate a dynamic regulation of circulating miRNA (ci-miRNA) levels following 0 hour (miR-106a, miR-221, miR-30b, miR-151-5p, let-7i, miR-146, miR-652 and miR-151-3p), 1 hour (miR-338-3p, miR-330-3p, miR-223, miR-139-5p and miR-143) and 3 hours (miR-1) after an acute exercise bout (P
U2 - 10.1371/journal.pone.0087308
DO - 10.1371/journal.pone.0087308
M3 - Journal article
C2 - 24586268
VL - 9
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 2
M1 - e87308
ER -
ID: 105481574