The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering. / Streck, S.; Clulow, Andrew J.; Nielsen, Hanne Mørck; Rades, Thomas; Boyd, B. J.; McDowell, Arlene.

In: Journal of Colloid and Interface Science, Vol. 555, 01.11.2019, p. 438-448.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Streck, S, Clulow, AJ, Nielsen, HM, Rades, T, Boyd, BJ & McDowell, A 2019, 'The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering', Journal of Colloid and Interface Science, vol. 555, pp. 438-448. https://doi.org/10.1016/j.jcis.2019.08.007

APA

Streck, S., Clulow, A. J., Nielsen, H. M., Rades, T., Boyd, B. J., & McDowell, A. (2019). The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering. Journal of Colloid and Interface Science, 555, 438-448. https://doi.org/10.1016/j.jcis.2019.08.007

Vancouver

Streck S, Clulow AJ, Nielsen HM, Rades T, Boyd BJ, McDowell A. The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering. Journal of Colloid and Interface Science. 2019 Nov 1;555:438-448. https://doi.org/10.1016/j.jcis.2019.08.007

Author

Streck, S. ; Clulow, Andrew J. ; Nielsen, Hanne Mørck ; Rades, Thomas ; Boyd, B. J. ; McDowell, Arlene. / The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering. In: Journal of Colloid and Interface Science. 2019 ; Vol. 555. pp. 438-448.

Bibtex

@article{42a4f1a8ba8d47edb799149612b065fc,

title = "The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering",

abstract = "Hypothesis: The distribution of three cell-penetrating peptides (CPPs) with different architectures (short, long linear and branched) on poly(lactic-co-glycolic) acid (PLGA) nanoparticles depends on the conjugation approach. Here, we explore the utilization of a zero-length crosslinking reaction for the covalent attachment of CPPs to PLGA nanoparticles and the translation of the reaction into a microfluidic platform. Experiments: A microfluidic device with a staggered herringbone mixer was used for the formulation of CPP-tagged PLGA nanoparticles. CPP-tagged PLGA nanoparticles were labeled with gold nanoparticles (AuNPs) and transmission electron microscopy (TEM) and small angle X-ray scattering (SAXS) were used to elucidate the distribution of CPPs. Findings: The SAXS scattering profiles for the CPP-tagged PLGA nanoparticles prepared with the in situ microfluidics conjugation approach indicated a distribution of the Au-labeled CPPs throughout the PLGA nanoparticles. For the post-microfluidics conjugation approach, the SAXS scattering profiles did not show the feature of the Au-labeled CPPs distributed throughout the PLGA nanoparticles and an arrangement of the Au-labeled CPP on the surface was support by TEM micrographs. The distribution of the CPPs was highly dependent on the conjugation approach and was not influenced by the architecture of the CPPs. The results provided insight for the rational design of CPP-tagged PLGA nanoparticles using microfluidics.",

keywords = "Branched cell-penetrating peptide, Microfluidics, PLGA nanoparticles, Small angle X-ray scattering, TAT, Transmission electron microscopy",

author = "S. Streck and Clulow, {Andrew J.} and Nielsen, {Hanne M{\o}rck} and Thomas Rades and Boyd, {B. J.} and Arlene McDowell",

year = "2019",

month = nov,

day = "1",

doi = "10.1016/j.jcis.2019.08.007",

language = "English",

volume = "555",

pages = "438--448",

journal = "Journal of Colloid and Interface Science",

issn = "0021-9797",

publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering

AU - Streck, S.

AU - Clulow, Andrew J.

AU - Nielsen, Hanne Mørck

AU - Rades, Thomas

AU - Boyd, B. J.

AU - McDowell, Arlene

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Hypothesis: The distribution of three cell-penetrating peptides (CPPs) with different architectures (short, long linear and branched) on poly(lactic-co-glycolic) acid (PLGA) nanoparticles depends on the conjugation approach. Here, we explore the utilization of a zero-length crosslinking reaction for the covalent attachment of CPPs to PLGA nanoparticles and the translation of the reaction into a microfluidic platform. Experiments: A microfluidic device with a staggered herringbone mixer was used for the formulation of CPP-tagged PLGA nanoparticles. CPP-tagged PLGA nanoparticles were labeled with gold nanoparticles (AuNPs) and transmission electron microscopy (TEM) and small angle X-ray scattering (SAXS) were used to elucidate the distribution of CPPs. Findings: The SAXS scattering profiles for the CPP-tagged PLGA nanoparticles prepared with the in situ microfluidics conjugation approach indicated a distribution of the Au-labeled CPPs throughout the PLGA nanoparticles. For the post-microfluidics conjugation approach, the SAXS scattering profiles did not show the feature of the Au-labeled CPPs distributed throughout the PLGA nanoparticles and an arrangement of the Au-labeled CPP on the surface was support by TEM micrographs. The distribution of the CPPs was highly dependent on the conjugation approach and was not influenced by the architecture of the CPPs. The results provided insight for the rational design of CPP-tagged PLGA nanoparticles using microfluidics.

AB - Hypothesis: The distribution of three cell-penetrating peptides (CPPs) with different architectures (short, long linear and branched) on poly(lactic-co-glycolic) acid (PLGA) nanoparticles depends on the conjugation approach. Here, we explore the utilization of a zero-length crosslinking reaction for the covalent attachment of CPPs to PLGA nanoparticles and the translation of the reaction into a microfluidic platform. Experiments: A microfluidic device with a staggered herringbone mixer was used for the formulation of CPP-tagged PLGA nanoparticles. CPP-tagged PLGA nanoparticles were labeled with gold nanoparticles (AuNPs) and transmission electron microscopy (TEM) and small angle X-ray scattering (SAXS) were used to elucidate the distribution of CPPs. Findings: The SAXS scattering profiles for the CPP-tagged PLGA nanoparticles prepared with the in situ microfluidics conjugation approach indicated a distribution of the Au-labeled CPPs throughout the PLGA nanoparticles. For the post-microfluidics conjugation approach, the SAXS scattering profiles did not show the feature of the Au-labeled CPPs distributed throughout the PLGA nanoparticles and an arrangement of the Au-labeled CPP on the surface was support by TEM micrographs. The distribution of the CPPs was highly dependent on the conjugation approach and was not influenced by the architecture of the CPPs. The results provided insight for the rational design of CPP-tagged PLGA nanoparticles using microfluidics.

KW - Branched cell-penetrating peptide

KW - Microfluidics

KW - PLGA nanoparticles

KW - Small angle X-ray scattering

KW - TAT

KW - Transmission electron microscopy

U2 - 10.1016/j.jcis.2019.08.007

DO - 10.1016/j.jcis.2019.08.007

M3 - Journal article

C2 - 31400536

AN - SCOPUS:85070889221

VL - 555

SP - 438

EP - 448

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

ID: 239817004