Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

Research output: Contribution to journalJournal articleResearchpeer-review

Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.

Original languageEnglish
JournalFEBS Letters
Issue number1-2
Pages (from-to)41-4
Number of pages4
Publication statusPublished - 2 Jan 2002

    Research areas

  • Chloramines, Drug Synergism, Hypochlorous Acid, Proteins, Reactive Nitrogen Species, Serum Albumin, Bovine, Superoxides

ID: 138279041