Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Purification of Functional Human TRP Channels Recombinantly Produced in Yeast. / Zhang, Liying; Wang, Kaituo; Klaerke, Dan Arne; Calloe, Kirstine; Lowrey, Lillian; Pedersen, Per Amstrup; Gourdon, Pontus; Gotfryd, Kamil.

In: Cells, Vol. 8, No. 2, 148, 2019, p. 1-20.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, L, Wang, K, Klaerke, DA, Calloe, K, Lowrey, L, Pedersen, PA, Gourdon, P & Gotfryd, K 2019, 'Purification of Functional Human TRP Channels Recombinantly Produced in Yeast', Cells, vol. 8, no. 2, 148, pp. 1-20. https://doi.org/10.3390/cells8020148

APA

Zhang, L., Wang, K., Klaerke, D. A., Calloe, K., Lowrey, L., Pedersen, P. A., Gourdon, P., & Gotfryd, K. (2019). Purification of Functional Human TRP Channels Recombinantly Produced in Yeast. Cells, 8(2), 1-20. [148]. https://doi.org/10.3390/cells8020148

Vancouver

Zhang L, Wang K, Klaerke DA, Calloe K, Lowrey L, Pedersen PA et al. Purification of Functional Human TRP Channels Recombinantly Produced in Yeast. Cells. 2019;8(2):1-20. 148. https://doi.org/10.3390/cells8020148

Author

Zhang, Liying ; Wang, Kaituo ; Klaerke, Dan Arne ; Calloe, Kirstine ; Lowrey, Lillian ; Pedersen, Per Amstrup ; Gourdon, Pontus ; Gotfryd, Kamil. / Purification of Functional Human TRP Channels Recombinantly Produced in Yeast. In: Cells. 2019 ; Vol. 8, No. 2. pp. 1-20.

Bibtex

@article{566d3b4d4372403a86a89700e646e228,
title = "Purification of Functional Human TRP Channels Recombinantly Produced in Yeast",
abstract = "(1) Background: Human transient receptor potential (TRP) channels constitute a large family of ion-conducting membrane proteins that allow the sensation of environmental cues. As the dysfunction of TRP channels contributes to the pathogenesis of many widespread diseases, including cardiac disorders, these proteins also represent important pharmacological targets. TRP channels are typically produced using expensive and laborious mammalian or insect cell-based systems. (2) Methods: We demonstrate an alternative platform exploiting the yeast Saccharomyces cerevisiae capable of delivering high yields of functional human TRP channels. We produce 11 full-length human TRP members originating from four different subfamilies, purify a selected subset of these to a high homogeneity and confirm retained functionality using TRPM8 as a model target. (3) Results: Our findings demonstrate the potential of the described production system for future functional, structural and pharmacological studies of human TRP channels.",
author = "Liying Zhang and Kaituo Wang and Klaerke, {Dan Arne} and Kirstine Calloe and Lillian Lowrey and Pedersen, {Per Amstrup} and Pontus Gourdon and Kamil Gotfryd",
year = "2019",
doi = "10.3390/cells8020148",
language = "English",
volume = "8",
pages = "1--20",
journal = "Cells",
issn = "2073-4409",
publisher = "MDPI AG",
number = "2",

}

RIS

TY - JOUR

T1 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

AU - Zhang, Liying

AU - Wang, Kaituo

AU - Klaerke, Dan Arne

AU - Calloe, Kirstine

AU - Lowrey, Lillian

AU - Pedersen, Per Amstrup

AU - Gourdon, Pontus

AU - Gotfryd, Kamil

PY - 2019

Y1 - 2019

N2 - (1) Background: Human transient receptor potential (TRP) channels constitute a large family of ion-conducting membrane proteins that allow the sensation of environmental cues. As the dysfunction of TRP channels contributes to the pathogenesis of many widespread diseases, including cardiac disorders, these proteins also represent important pharmacological targets. TRP channels are typically produced using expensive and laborious mammalian or insect cell-based systems. (2) Methods: We demonstrate an alternative platform exploiting the yeast Saccharomyces cerevisiae capable of delivering high yields of functional human TRP channels. We produce 11 full-length human TRP members originating from four different subfamilies, purify a selected subset of these to a high homogeneity and confirm retained functionality using TRPM8 as a model target. (3) Results: Our findings demonstrate the potential of the described production system for future functional, structural and pharmacological studies of human TRP channels.

AB - (1) Background: Human transient receptor potential (TRP) channels constitute a large family of ion-conducting membrane proteins that allow the sensation of environmental cues. As the dysfunction of TRP channels contributes to the pathogenesis of many widespread diseases, including cardiac disorders, these proteins also represent important pharmacological targets. TRP channels are typically produced using expensive and laborious mammalian or insect cell-based systems. (2) Methods: We demonstrate an alternative platform exploiting the yeast Saccharomyces cerevisiae capable of delivering high yields of functional human TRP channels. We produce 11 full-length human TRP members originating from four different subfamilies, purify a selected subset of these to a high homogeneity and confirm retained functionality using TRPM8 as a model target. (3) Results: Our findings demonstrate the potential of the described production system for future functional, structural and pharmacological studies of human TRP channels.

U2 - 10.3390/cells8020148

DO - 10.3390/cells8020148

M3 - Journal article

C2 - 30754715

VL - 8

SP - 1

EP - 20

JO - Cells

JF - Cells

SN - 2073-4409

IS - 2

M1 - 148

ER -

ID: 213713237