Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour

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We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.

Original languageEnglish
Article number46
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Pages (from-to)306-312
Number of pages7
Publication statusPublished - 21 Jul 2005
EventImaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States
Duration: 24 Jan 200527 Jan 2005


ConferenceImaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
CountryUnited States
CitySan Jose, CA

    Research areas

  • Generalized phase contrast, Manipulation of living cells, Real-time multi-beam optical trapping

ID: 225839121