TY - JOUR

T1 - No effect of dietary fish oil supplementation on the recruitment of brown and brite adipocytes in mice or humans under thermoneutral conditions

AU - Maurer, Stefanie F

AU - Dieckmann, Sebastian

AU - Lund, Jens

AU - Fromme, Tobias

AU - Hess, Anne Lundby

AU - Colson, Cécilia

AU - Kjølbæk, Louise

AU - Astrup, Arne

AU - Gillum, Matthew Paul

AU - Larsen, Lesli Hingstrup

AU - Liebisch, Gerhard

AU - Amri, Ez-Zoubir

AU - Klingenspor, Martin

N1 - CURIS 2021 NEXS 034

PY - 2021

Y1 - 2021

N2 - Scope: Brown and brite adipocytes within the mammalian adipose organ provide non-shivering thermogenesis and thus, have an exceptional capacity to dissipate chemical energy as heat. Polyunsaturated fatty acids of the n3-series, abundant in fish oil, have been repeatedly demonstrated to enhance the recruitment of thermogenic capacity in these cells, consequently affecting body adiposity and glucose tolerance. We scrutinized these effects in mice housed in a thermoneutral environment and in a human dietary intervention trial.Methods and results: Mice were housed in a thermoneutral environment eliminating the superimposing effect of mild cold-exposure on thermogenic adipocyte recruitment. Dietary fish oil supplementation in two different inbred mouse strains neither affected body mass trajectory nor enhanced the recruitment of brown and brite adipocytes, both in the presence and absence of a β3 adrenoreceptor agonist imitating the effect of cold-exposure on adipocytes. In line with these findings, dietary fish oil supplementation of persons with overweight or obesity failed to recruit thermogenic adipocytes in subcutaneous adipose tissue.Conclusion: Thus, our data question the hypothesized potential of n3-polyunsaturated fatty acids as modulators of adipocyte-based thermogenesis and energy balance regulation. This article is protected by copyright. All rights reserved.

AB - Scope: Brown and brite adipocytes within the mammalian adipose organ provide non-shivering thermogenesis and thus, have an exceptional capacity to dissipate chemical energy as heat. Polyunsaturated fatty acids of the n3-series, abundant in fish oil, have been repeatedly demonstrated to enhance the recruitment of thermogenic capacity in these cells, consequently affecting body adiposity and glucose tolerance. We scrutinized these effects in mice housed in a thermoneutral environment and in a human dietary intervention trial.Methods and results: Mice were housed in a thermoneutral environment eliminating the superimposing effect of mild cold-exposure on thermogenic adipocyte recruitment. Dietary fish oil supplementation in two different inbred mouse strains neither affected body mass trajectory nor enhanced the recruitment of brown and brite adipocytes, both in the presence and absence of a β3 adrenoreceptor agonist imitating the effect of cold-exposure on adipocytes. In line with these findings, dietary fish oil supplementation of persons with overweight or obesity failed to recruit thermogenic adipocytes in subcutaneous adipose tissue.Conclusion: Thus, our data question the hypothesized potential of n3-polyunsaturated fatty acids as modulators of adipocyte-based thermogenesis and energy balance regulation. This article is protected by copyright. All rights reserved.

KW - Faculty of Science

KW - Adipose tissue

KW - Ucp1

KW - Thermogenesis

KW - PUFA

KW - n6

KW - n3

KW - Fish oil

U2 - 10.1002/mnfr.202000681

DO - 10.1002/mnfr.202000681

M3 - Journal article

C2 - 33274552

VL - 65

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 2

M1 - 2000681

ER -