Nitric oxide (NO) production in mammalian non-tumorigenic epithelial cells of the small intestine and macrophages induced by individual strains of lactobacilli and bifidobacteria

Research output: Contribution to journalJournal articleResearchpeer-review

  • Natasa Pipenbaher
  • Peter Lange Møller
  • Jan Dolinsek
  • Mogens Jakobsen
  • Hana Weingartl
  • Avrelija Cencic

Nitric oxide (NO) affects multiple gastrointestinal functions, including mucosal inflammation and antimicrobial activity. The aim of this study was to screen the ability of probiotic bacteria to stimulate NO production in porcine intestinal epithelial cells and macrophages in the presence and absence of interferon gamma (INF-¿). Production of NO in intestinal epithelium was stimulated by individual strains of lactobacilli without INF-¿ priming. While none of the tested bifidobacteria were capable of inducing NO production, most constitutively secreted NO. Most tested strains induced a significant increase in NO production compared with the control cells in the macrophage cell line 3D4/21. Results support the protective role of the individual strains of the genera Lactobacillus and Bifidobacterium and may lead to new approaches for manipulating and regulating immune responses at the mucosal surfaces of the gastrointestinal tract.

Original languageEnglish
JournalInternational Dairy Journal
Issue number3
Pages (from-to)166-171
Number of pages6
Publication statusPublished - 2009

ID: 9199192