Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status
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Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status. / Persson, Daniel Olof; de Bang, Thomas Christian; Pedas, Pai Rosager; Kutman, Umit Baris; Cakmak, Ismail; Andersen, Birgit; Finnie, Christine; Schjørring, Jan Kofod; Husted, Søren.
In: New Phytologist, Vol. 211, No. 4, 2016, p. 1255-1265.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status
AU - Persson, Daniel Olof
AU - de Bang, Thomas Christian
AU - Pedas, Pai Rosager
AU - Kutman, Umit Baris
AU - Cakmak, Ismail
AU - Andersen, Birgit
AU - Finnie, Christine
AU - Schjørring, Jan Kofod
AU - Husted, Søren
N1 - © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
PY - 2016
Y1 - 2016
N2 - Low concentration of zinc (Zn) in the endosperm of cereals is a major factor contributing to Zn deficiency in human populations. We have investigated how combined Zn and nitrogen (N) fertilization affects the speciation and localization of Zn in durum wheat (Triticum durum). Zn-binding proteins were analysed with liquid chromatography ICP-MS and Orbitrap MS(2) , respectively. Laser ablation ICP-MS with simultaneous Zn, sulphur (S) and phosphorus (P) detection was used for bioimaging of Zn and its potential ligands. Increasing the Zn and N supply had a major impact on the Zn concentration in the endosperm, reaching concentrations higher than current breeding targets. The S concentration also increased, but S was only partly co-localized with Zn. The mutual Zn and S enrichment was reflected in substantially more Zn bound to small cysteine-rich proteins (apparent size 10-30 kDa), whereas the response of larger proteins (apparent size > 50 kDa) was only modest. Most of the Zn-responsive proteins were associated with redox- and stress-related processes. This study offers a methodological platform to deepen the understanding of processes behind endosperm Zn enrichment. Novel information is provided on how the localization and speciation of Zn is modified during Zn biofortification of grains.
AB - Low concentration of zinc (Zn) in the endosperm of cereals is a major factor contributing to Zn deficiency in human populations. We have investigated how combined Zn and nitrogen (N) fertilization affects the speciation and localization of Zn in durum wheat (Triticum durum). Zn-binding proteins were analysed with liquid chromatography ICP-MS and Orbitrap MS(2) , respectively. Laser ablation ICP-MS with simultaneous Zn, sulphur (S) and phosphorus (P) detection was used for bioimaging of Zn and its potential ligands. Increasing the Zn and N supply had a major impact on the Zn concentration in the endosperm, reaching concentrations higher than current breeding targets. The S concentration also increased, but S was only partly co-localized with Zn. The mutual Zn and S enrichment was reflected in substantially more Zn bound to small cysteine-rich proteins (apparent size 10-30 kDa), whereas the response of larger proteins (apparent size > 50 kDa) was only modest. Most of the Zn-responsive proteins were associated with redox- and stress-related processes. This study offers a methodological platform to deepen the understanding of processes behind endosperm Zn enrichment. Novel information is provided on how the localization and speciation of Zn is modified during Zn biofortification of grains.
KW - Journal Article
U2 - 10.1111/nph.13989
DO - 10.1111/nph.13989
M3 - Journal article
C2 - 27159614
VL - 211
SP - 1255
EP - 1265
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 4
ER -
ID: 169134791