Methods to classify bacterial pathogens in cystic fibrosis

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Standard

Methods to classify bacterial pathogens in cystic fibrosis. / Bjarnsholt, Thomas; Nielsen, Xiaohui Chen; Johansen, Ulla; Nørgaard, Lena; Høiby, Niels.

In: Methods in Molecular Biology, Vol. 742, 2011, p. 143-71.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bjarnsholt, T, Nielsen, XC, Johansen, U, Nørgaard, L & Høiby, N 2011, 'Methods to classify bacterial pathogens in cystic fibrosis', Methods in Molecular Biology, vol. 742, pp. 143-71. https://doi.org/10.1007/978-1-61779-120-8_9

APA

Bjarnsholt, T., Nielsen, X. C., Johansen, U., Nørgaard, L., & Høiby, N. (2011). Methods to classify bacterial pathogens in cystic fibrosis. Methods in Molecular Biology, 742, 143-71. https://doi.org/10.1007/978-1-61779-120-8_9

Vancouver

Bjarnsholt T, Nielsen XC, Johansen U, Nørgaard L, Høiby N. Methods to classify bacterial pathogens in cystic fibrosis. Methods in Molecular Biology. 2011;742:143-71. https://doi.org/10.1007/978-1-61779-120-8_9

Author

Bjarnsholt, Thomas ; Nielsen, Xiaohui Chen ; Johansen, Ulla ; Nørgaard, Lena ; Høiby, Niels. / Methods to classify bacterial pathogens in cystic fibrosis. In: Methods in Molecular Biology. 2011 ; Vol. 742. pp. 143-71.

Bibtex

@article{d70bbf7eb6bb4a19b5dd257bc727c1ca,
title = "Methods to classify bacterial pathogens in cystic fibrosis",
abstract = "Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch's criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.",
author = "Thomas Bjarnsholt and Nielsen, {Xiaohui Chen} and Ulla Johansen and Lena N{\o}rgaard and Niels H{\o}iby",
year = "2011",
doi = "10.1007/978-1-61779-120-8_9",
language = "English",
volume = "742",
pages = "143--71",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Methods to classify bacterial pathogens in cystic fibrosis

AU - Bjarnsholt, Thomas

AU - Nielsen, Xiaohui Chen

AU - Johansen, Ulla

AU - Nørgaard, Lena

AU - Høiby, Niels

PY - 2011

Y1 - 2011

N2 - Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch's criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.

AB - Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch's criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.

U2 - 10.1007/978-1-61779-120-8_9

DO - 10.1007/978-1-61779-120-8_9

M3 - Journal article

C2 - 21547731

VL - 742

SP - 143

EP - 171

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 33910239