Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal. / Fazakerley, Daniel J; Fritzen, Andreas Mæchel; Nelson, Marin E; Thorius, Ida H; Cooke, Kristen C; Humphrey, Sean J; Cooney, Gregory J; James, David E.

In: Bio-protocol, Vol. 9, No. 2, e3146, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Fazakerley, DJ, Fritzen, AM, Nelson, ME, Thorius, IH, Cooke, KC, Humphrey, SJ, Cooney, GJ & James, DE 2019, 'Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal', Bio-protocol, vol. 9, no. 2, e3146. https://doi.org/10.21769/BioProtoc.3146

APA

Fazakerley, D. J., Fritzen, A. M., Nelson, M. E., Thorius, I. H., Cooke, K. C., Humphrey, S. J., ... James, D. E. (2019). Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal. Bio-protocol, 9(2), [e3146]. https://doi.org/10.21769/BioProtoc.3146

Vancouver

Fazakerley DJ, Fritzen AM, Nelson ME, Thorius IH, Cooke KC, Humphrey SJ et al. Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal. Bio-protocol. 2019;9(2). e3146. https://doi.org/10.21769/BioProtoc.3146

Author

Fazakerley, Daniel J ; Fritzen, Andreas Mæchel ; Nelson, Marin E ; Thorius, Ida H ; Cooke, Kristen C ; Humphrey, Sean J ; Cooney, Gregory J ; James, David E. / Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal. In: Bio-protocol. 2019 ; Vol. 9, No. 2.

Bibtex

@article{2317c616d38743d091861150dfca45a3,
title = "Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal",
abstract = "Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.",
keywords = "Faculty of Science, Insulin, Adipose tissue, Muscle, Glucose transport, Glucose uptake, Insulin tolerance test",
author = "Fazakerley, {Daniel J} and Fritzen, {Andreas M{\ae}chel} and Nelson, {Marin E} and Thorius, {Ida H} and Cooke, {Kristen C} and Humphrey, {Sean J} and Cooney, {Gregory J} and James, {David E}",
note = "CURIS 2019 NEXS 050",
year = "2019",
doi = "10.21769/BioProtoc.3146",
language = "English",
volume = "9",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "2",

}

RIS

TY - JOUR

T1 - Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

AU - Fazakerley, Daniel J

AU - Fritzen, Andreas Mæchel

AU - Nelson, Marin E

AU - Thorius, Ida H

AU - Cooke, Kristen C

AU - Humphrey, Sean J

AU - Cooney, Gregory J

AU - James, David E

N1 - CURIS 2019 NEXS 050

PY - 2019

Y1 - 2019

N2 - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

AB - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

KW - Faculty of Science

KW - Insulin

KW - Adipose tissue

KW - Muscle

KW - Glucose transport

KW - Glucose uptake

KW - Insulin tolerance test

U2 - 10.21769/BioProtoc.3146

DO - 10.21769/BioProtoc.3146

M3 - Journal article

VL - 9

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 2

M1 - e3146

ER -

ID: 213166605