Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides

Research output: Contribution to journal › Journal article › peer-review

Standard

Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides. / Hampton, Mark B; Morgan, Philip E; Davies, Michael Jonathan.

In: FEBS Letters, Vol. 527, No. 1-3, 11.09.2002, p. 289-92.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Hampton, MB, Morgan, PE & Davies, MJ 2002, 'Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides', FEBS Letters, vol. 527, no. 1-3, pp. 289-92.

APA

Hampton, M. B., Morgan, P. E., & Davies, M. J. (2002). Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides. FEBS Letters, 527(1-3), 289-92.

Vancouver

Hampton MB, Morgan PE, Davies MJ. Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides. FEBS Letters. 2002 Sep 11;527(1-3):289-92.

Author

Hampton, Mark B ; Morgan, Philip E ; Davies, Michael Jonathan. / Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides. In: FEBS Letters. 2002 ; Vol. 527, No. 1-3. pp. 289-92.

Bibtex

@article{e572ab0c783d4dc9991bdefbb681fc00,

title = "Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides",

abstract = "Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.",

keywords = "Amino Acids, Caspase Inhibitors, Cysteine Proteinase Inhibitors, Dose-Response Relationship, Drug, Enzyme Activation, Humans, Hydrogen Peroxide, Jurkat Cells, Peroxides, Tryptophan, Tyrosine",

author = "Hampton, {Mark B} and Morgan, {Philip E} and Davies, {Michael Jonathan}",

year = "2002",

month = sep,

day = "11",

language = "English",

volume = "527",

pages = "289--92",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "1-3",

}

RIS

TY - JOUR

T1 - Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides

AU - Hampton, Mark B

AU - Morgan, Philip E

AU - Davies, Michael Jonathan

PY - 2002/9/11

Y1 - 2002/9/11

N2 - Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.

AB - Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.

KW - Amino Acids

KW - Caspase Inhibitors

KW - Cysteine Proteinase Inhibitors

KW - Dose-Response Relationship, Drug

KW - Enzyme Activation

KW - Humans

KW - Hydrogen Peroxide

KW - Jurkat Cells

KW - Peroxides

KW - Tryptophan

KW - Tyrosine

M3 - Journal article

C2 - 12220676

VL - 527

SP - 289

EP - 292

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1-3

ER -

ID: 138276610